論文 - 詳細
| RRC ID | 11166 |
|---|---|
| 著者 | Doi N, Zenno S, Ueda R, Ohki-Hamazaki H, Ui-Tei K, Saigo K. |
| タイトル | Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors. |
| ジャーナル | Curr Biol |
| Abstract |
RNA interference (RNAi) is the process of long, double-stranded (ds), RNA-dependent posttranscriptional gene silencing (PTGS). In lower eukaryotes, dsRNA introduced into the cytoplasm is cleaved by the RNaseIII-like enzyme, Dicer, to 21-23 nt RNA (short interfering [si] RNA), which may serve as guide for target mRNA degradation. In mammals, long-dsRNA-dependent PTGS is applicable only to a limited number of cell types, whereas siRNA synthesized in vitro is capable of effectively inducing gene silencing in a wide variety of cells. Although biochemical and genetic analyses in lower eukaryotes showed that Dicer and some PIWI family member proteins are essential for long-dsRNA-dependent PTGS, little is known about the molecular mechanisms underlying siRNA-based PTGS. Here, we show that Dicer and eIF2C translation initiation factors belonging to the PIWI family (eIF2C1-4) play an essential role in mammalian siRNA-mediated PTGS, most probably through synergistic interactions. Immunoprecipitation experiments suggest that, in human and mouse cells, complex formation occurs between Dicer and eIF2C1 or 2 and that the PIWI domain of eIF2C is essential for the formation of this complex. |
| 巻・号 | 13(1) |
| ページ | 41-6 |
| 公開日 | 2003-1-8 |
| DOI | 10.1016/s0960-9822(02)01394-5 |
| PII | S0960-9822(02)01394-5 |
| PMID | 12526743 |
| MeSH | 3T3 Cells Amino Acid Sequence Animals Argonaute Proteins Endoribonucleases / genetics Endoribonucleases / metabolism* Eukaryotic Initiation Factors* Gene Silencing / physiology* Genes, myc HeLa Cells Humans Macromolecular Substances Mammals / genetics Mice Molecular Sequence Data Peptide Initiation Factors / genetics Peptide Initiation Factors / metabolism* Precipitin Tests Protein Biosynthesis / physiology RNA Interference RNA, Small Interfering / genetics RNA, Small Interfering / physiology* Ribonuclease III |
| IF | 9.601 |
| 引用数 | 161 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
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オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 42 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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