論文 - 詳細
| RRC ID | 11172 |
|---|---|
| 著者 | Sasaki Y, Matsui K, Aoyama Y, Kikuchi J. |
| タイトル | Cerasome as an infusible and cell-friendly gene carrier: synthesis of cerasome-forming lipids and transfection using cerasome. |
| ジャーナル | Nat Protoc |
| Abstract |
Sonication of a pre-agitated aqueous solution of cationic lipid having a (EtO)3SiCH2CH2CH2 group on the quarternized ammonium nitrogen results in partially silica- or ceramic-coated liposome (cerasome), which can be used as an excellent transfection agent. Non-silylated reference lipid, which may represent cationic lipids that are used in conventional lipofection experiments, form a compact liposome, which undergoes DNA-induced fusion to provide transfection-irrelevant and larger (100-300 nm), more toxic particles. The surface-rigidified cerasome is infusible and the monomeric cerasome complex of DNA is of viral size (approximately 70 nm) and exhibits a remarkable transfection performance with a 10(2)-10(3)-fold higher efficiency (relative to the non-silylated reference lipid), minimized cytotoxicity and serum compatibility. The cerasome lipid is obtained by the reaction of 3-bromopropyltriethoxysilane with a tertiary amine derivative of the lipid. Preparation of an aqueous cerasome solution takes 1-2 h. The cerasome-DNA complex and the transfection takes about 3 d to complete. |
| 巻・号 | 1(3) |
| ページ | 1227-34 |
| 公開日 | 2006-1-1 |
| DOI | 10.1038/nprot.2006.182 |
| PII | nprot.2006.182 |
| PMID | 17406406 |
| MeSH | Ceramics / chemistry* DNA / administration & dosage DNA / metabolism* Liposomes / chemical synthesis* Transfection / methods* |
| IF | 10.419 |
| 引用数 | 30 |
| WOS 分野 | BIOCHEMICAL RESEARCH METHODS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |