| RRC ID |
11172
|
| 著者 |
Sasaki Y, Matsui K, Aoyama Y, Kikuchi J.
|
| タイトル |
Cerasome as an infusible and cell-friendly gene carrier: synthesis of cerasome-forming lipids and transfection using cerasome.
|
| ジャーナル |
Nat Protoc
|
| Abstract |
Sonication of a pre-agitated aqueous solution of cationic lipid having a (EtO)3SiCH2CH2CH2 group on the quarternized ammonium nitrogen results in partially silica- or ceramic-coated liposome (cerasome), which can be used as an excellent transfection agent. Non-silylated reference lipid, which may represent cationic lipids that are used in conventional lipofection experiments, form a compact liposome, which undergoes DNA-induced fusion to provide transfection-irrelevant and larger (100-300 nm), more toxic particles. The surface-rigidified cerasome is infusible and the monomeric cerasome complex of DNA is of viral size (approximately 70 nm) and exhibits a remarkable transfection performance with a 10(2)-10(3)-fold higher efficiency (relative to the non-silylated reference lipid), minimized cytotoxicity and serum compatibility. The cerasome lipid is obtained by the reaction of 3-bromopropyltriethoxysilane with a tertiary amine derivative of the lipid. Preparation of an aqueous cerasome solution takes 1-2 h. The cerasome-DNA complex and the transfection takes about 3 d to complete.
|
| 巻・号 |
1(3)
|
| ページ |
1227-34
|
| 公開日 |
2006-1-1
|
| DOI |
10.1038/nprot.2006.182
|
| PII |
nprot.2006.182
|
| PMID |
17406406
|
| MeSH |
Ceramics / chemistry*
DNA / administration & dosage
DNA / metabolism*
Liposomes / chemical synthesis*
Transfection / methods*
|
| IF |
10.419
|
| 引用数 |
30
|
|
WOS 分野
|
BIOCHEMICAL RESEARCH METHODS
|
| リソース情報 |
| ヒト・動物細胞 |
HeLa(RCB0007) |
