論文 - 詳細
| RRC ID | 11173 |
|---|---|
| 著者 | Engelhardt P, Meriläinen J, Zhao F, Uchiyama S, Fukui K, Lehto VP. |
| タイトル | Whole-mount immunoelectron tomography of chromosomes and cells. |
| ジャーナル | Methods Mol Biol |
| Abstract |
Standard immunogold-labeling methods in transmission electron microscopy (TEM) are unable to locate immunogold particles in the depth direction. This inability does not only concern bulky whole mounts, but also sections. A partial solution to the problem is stereo inspection. However, three-dimensional reconstruction of immunogold-labeled structures, that is, immuno-electron tomography (IET), is a correct solution for this inconsistency. Striking improvement in resolution is achieved: the 1.4-nm immunogold particles are shown in IET that are not detected in the original tilt series. IET is not restricted to laboratories with advanced medium- or high-voltage TEM and super-computing facilities; the methods we have developed for whole-mounted chromosomes and also for whole-mounted cytoskeleton of fibroblasts work remarkably well with ordinary 80-kV TEMs equipped with a goniometer to collect tilt series for IET on film. In addition, free programs are available to produce three-dimensional reconstructions even without high-performance computers. These improvements make it possible to many laboratories without modern facilities to perform IET reconstruction with standard TEM apparatus. |
| 巻・号 | 369 |
| ページ | 387-405 |
| 公開日 | 2007-1-1 |
| DOI | 10.1007/978-1-59745-294-6_19 |
| PII | 1-59745-294-7:387 |
| PMID | 17656761 |
| MeSH | Animals Cell Line Cells / ultrastructure Chick Embryo Chromosomes / ultrastructure* Chromosomes, Human / ultrastructure Gold Colloid HeLa Cells Humans Imaging, Three-Dimensional / methods* Microscopy, Immunoelectron / methods* Tomography / methods |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) HeLa.S3(RCB0191) K562(RCB0027) BALL-1(RCB0256) |