| RRC ID |
11174
|
| 著者 |
Tao Y, Nomura M, Kitabatake N, Tani F.
|
| タイトル |
Mouse CD40-transfected cell lines cannot exhibit the binding and RANTES-stimulating activity of exogenous heat shock protein 70.
|
| ジャーナル |
Mol Immunol
|
| Abstract |
Here we demonstrate the inducible mouse Hsp72 binds markedly to lymphoid neoplastic macrophage-like P388D1 cells. To examine whether mouse CD40 can play a role in signaling exogenously administered HSP70 in a fashion similar to that of human CD40, we established mouse CD40-transfectants of both human 293 cells and murine-pro-B cell line Ba/F3. A small portion of mouse CD40 expressed on 293-derived transfectants was the mature form with a signal-transducible C-terminal domain, whereas a majority of expressed antigen showed the molecular size smaller than we expect. Flow cytometry showed that mouse Hsp72, but neither its deletion variants nor the related Escherichia coli DnaK, bound to the 293-derived transfectants regardless of CD40 expression. CD40 molecules expressed on the transfectants showed the binding of soluble form of CD40L but this binding was not inhibited by excess amount of HSP70. CD40L, but not any HSP70 recombinant proteins, stimulated the production of chemokine RANTES in the transfectants. Furthermore, no RANTES production was induced by HSP70-RCMLA complex in the transfectants, although it binds to 293-derived cells in a CD40-independent manner. No interaction between mouse CD40 and HSP70 recombinant proteins was detected by using the Ba/F3-derived transfectants that express the mature form of mouse CD40. The present results imply that mouse CD40 expressed on the transfectants differs from its human homolog in the binding of exogenously administered HSP70.
|
| 巻・号 |
44(6)
|
| ページ |
1262-73
|
| 公開日 |
2007-2-1
|
| DOI |
10.1016/j.molimm.2006.06.002
|
| PII |
S0161-5890(06)00208-2
|
| PMID |
16930707
|
| MeSH |
Animals
CD40 Antigens / genetics*
CD40 Antigens / metabolism*
CD40 Antigens / physiology
Cell Line
Cell Line, Tumor
Chemokine CCL5 / genetics
Chemokine CCL5 / metabolism*
HSP70 Heat-Shock Proteins / metabolism
HSP70 Heat-Shock Proteins / physiology*
HSP72 Heat-Shock Proteins / metabolism
Humans
Leukemia P388 / immunology
Leukemia P388 / metabolism*
Mice
Protein Binding / genetics
Protein Binding / immunology
Transfection*
|
| IF |
3.641
|
| 引用数 |
5
|
|
WOS 分野
|
IMMUNOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
Ba/F3(RCB0805)
WEHI-3(RCB0035)
293(RCB1637) |
