論文 - 詳細
| RRC ID | 11175 |
|---|---|
| 著者 | Okuyama T, Yamazoe H, Seto Y, Suzuki H, Fukuda J. |
| タイトル | Cell micropatterning inside a microchannel and assays under a stable concentration gradient. |
| ジャーナル | J Biosci Bioeng |
| Abstract |
We describe the use of a microfluidic device to micropattern cells in a microchannel and investigated the behavior of these cells under a concentration gradient. The microfluidic device consisted of 3 parts: a branched channel for generating a stable concentration gradient, a main channel for culturing cells, and 2 side channels that flowed into the main channel. The main channel was coated with a cross-linked albumin that was initially cell-repellent but that could become cell-adherent by electrostatic adsorption of a polycation. A sheath flow stream, which was generated by introducing a polycation solution from the branched channel and a buffer solution from the 2 side channels, was used to change a specific region in the main channel from cell-repellent to cell-adhesive. In this way, cells attached to the central region along the main channel. The remaining surface was subsequently changed to cell-adhesive, thereby facilitating cell migration from a fixed location under a concentration gradient. We demonstrated that with this device, the gradient generator could be used to conduct simultaneous cytotoxic assays with anticancer agents; further, by combining this device with cell micropatterning, migration assays under a concentration gradient of biological factors could be conducted. |
| 巻・号 | 110(2) |
| ページ | 230-7 |
| 公開日 | 2010-8-1 |
| DOI | 10.1016/j.jbiosc.2010.02.001 |
| PII | S1389-1723(10)00042-3 |
| PMID | 20547384 |
| MeSH | Biological Assay / instrumentation* Biosensing Techniques / instrumentation* Cell Culture Techniques / instrumentation* Cell Polarity Equipment Design Equipment Failure Analysis Flow Injection Analysis / instrumentation* Microarray Analysis / instrumentation* Microfluidic Analytical Techniques / instrumentation* Miniaturization Perfusion / instrumentation* |
| IF | 2.366 |
| 引用数 | 10 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | 3T3 Swiss Albino(RCB1642) Hep G2 PC-12(RCB0009) |