RRC ID 11175
著者 Okuyama T, Yamazoe H, Seto Y, Suzuki H, Fukuda J.
タイトル Cell micropatterning inside a microchannel and assays under a stable concentration gradient.
ジャーナル J Biosci Bioeng
Abstract We describe the use of a microfluidic device to micropattern cells in a microchannel and investigated the behavior of these cells under a concentration gradient. The microfluidic device consisted of 3 parts: a branched channel for generating a stable concentration gradient, a main channel for culturing cells, and 2 side channels that flowed into the main channel. The main channel was coated with a cross-linked albumin that was initially cell-repellent but that could become cell-adherent by electrostatic adsorption of a polycation. A sheath flow stream, which was generated by introducing a polycation solution from the branched channel and a buffer solution from the 2 side channels, was used to change a specific region in the main channel from cell-repellent to cell-adhesive. In this way, cells attached to the central region along the main channel. The remaining surface was subsequently changed to cell-adhesive, thereby facilitating cell migration from a fixed location under a concentration gradient. We demonstrated that with this device, the gradient generator could be used to conduct simultaneous cytotoxic assays with anticancer agents; further, by combining this device with cell micropatterning, migration assays under a concentration gradient of biological factors could be conducted.
巻・号 110(2)
ページ 230-7
公開日 2010-8-1
DOI 10.1016/j.jbiosc.2010.02.001
PII S1389-1723(10)00042-3
PMID 20547384
MeSH Biological Assay / instrumentation* Biosensing Techniques / instrumentation* Cell Culture Techniques / instrumentation* Cell Polarity Equipment Design Equipment Failure Analysis Flow Injection Analysis / instrumentation* Microarray Analysis / instrumentation* Microfluidic Analytical Techniques / instrumentation* Miniaturization Perfusion / instrumentation*
IF 2.366
引用数 10
WOS 分野 FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY
リソース情報
ヒト・動物細胞 3T3 Swiss Albino(RCB1642) Hep G2 PC-12(RCB0009)