RRC ID 11178
Author Seo JH, Matsuno R, Takai M, Ishihara K.
Title Cell adhesion on phase-separated surface of block copolymer composed of poly(2-methacryloyloxyethyl phosphorylcholine) and poly(dimethylsiloxane).
Journal Biomaterials
Abstract We investigated the morphological effect of phase-separated block copolymer surfaces composed of poly(2-methacryloyloxyethyl phosphorylcholine (MPC)) (PMPC) and poly(dimethylsiloxane) (PDMS) on protein adsorption and cell adhesion behavior. We observed three different types of phase-separated surface morphologies by TEM and AFM. The elemental composition of phosphorus on the surface increases with the PMPC composition. Furthermore, the polymer surface formed by a block copolymer-containing a higher MPC unit composition shows a slightly lower static water contact angle. This result indicates that the elemental surface ratio of the surface depends on the MPC composition in the block copolymer. Protein adsorption tests revealed that only hydrophobic PDMS domains showed selective protein adsorption. Cell adhesion tests revealed that the number of adhered cells increased with increasing hydrophobic PDMS domain size of block copolymers in serum-containing media. In contrast, no cells adhered onto block copolymer surfaces in serum-free media, whereas a large amount of adhered cells were observed on the hydrophobic PDMS surface. This result indicates that segregated hydrophobic domains on a biocompatible PMPC surface strongly affect serum protein adsorption, thereby promoting considerable cell adhesion, although the surface is hydrophilic. Thus, both the composition of MPC units and the segregated hydrophobic surface morphology are important considerations in biomaterial surface design.
Volume 30(29)
Pages 5330-40
Published 2009-10
DOI 10.1016/j.biomaterials.2009.06.031
PII S0142-9612(09)00634-6
PMID 19592090
MeSH Animals Biocompatible Materials / chemistry* Cell Adhesion / physiology Cell Line Dimethylpolysiloxanes / chemistry* Fibroblasts / cytology* Fibroblasts / physiology* Materials Testing Methacrylates / chemistry* Mice Phase Transition Phosphorylcholine / analogs & derivatives* Phosphorylcholine / chemistry Polymethacrylic Acids
IF 8.402
Times Cited 38
Human and Animal Cells L929 (RCB0081)