| RRC ID |
11181
|
| 著者 |
Roy MK, Nakahara K, Na TV, Trakoontivakorn G, Takenaka M, Isobe S, Tsushida T.
|
| タイトル |
Baicalein, a flavonoid extracted from a methanolic extract of Oroxylum indicum inhibits proliferation of a cancer cell line in vitro via induction of apoptosis.
|
| ジャーナル |
Pharmazie
|
| Abstract |
A methanolic extract of the fruits of Oroxylum indicum, which is widely used in traditional Chinese herbal medicine for its anti-inflammatory, anti-pyretic and anti-hypersensitivity effects, inhibited in vitro proliferation of HL-60 cells. The flavonoid baicalein was found as an active component in the extract. Analysis of freeze-dried fruits of the plant indicated that this component comprised about 4% of the material by dry weight. In this study, we investigated the in vitro effects of baicalein on the viability and induction of apoptosis in the HL-60 cell line. The cell viability after treating with baicalein for 24 h was quantified by counting viable cells using trypan blue staining. The results showed that baicalein caused a 50% inhibition of HL-60 cells at concentrations of 25-30 microM. The inhibition of proliferation of HL-60 cells due to 36-48 h exposure to 10 or 20 microM baicalein was associated with the accumulation of cells at S or G2M phases. However, proliferation inhibition at a higher dose may be associated with induction by apoptosis, as evidenced by the typical nuclear fragmentation using DNA fragmentation assay and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL). The results indicate that baicalein has anti-tumor effects on human cancer cells, and Oroxylum indicum extract could be used in supplementary cancer therapy.
|
| 巻・号 |
62(2)
|
| ページ |
149-53
|
| 公開日 |
2007-2-1
|
| PMID |
17341037
|
| MeSH |
Antineoplastic Agents, Phytogenic / pharmacology*
Apoptosis / drug effects*
Cell Cycle
Cell Line, Tumor
Cell Survival
Chromatography, High Pressure Liquid
DNA Fragmentation / drug effects
Flavanones / pharmacology*
Flow Cytometry
HL-60 Cells
Humans
In Situ Nick-End Labeling
Methanol
Plant Extracts / chemistry
Plant Extracts / pharmacology
Plants, Medicinal / chemistry
Solvents
Spectrophotometry, Ultraviolet
|
| IF |
1.198
|
| 引用数 |
55
|
|
WOS 分野
|
CHEMISTRY, MEDICINAL
PHARMACOLOGY & PHARMACY
CHEMISTRY, MULTIDISCIPLINARY
|
| リソース情報 |
| ヒト・動物細胞 |
HL60(RCB0041) |
