| RRC ID |
11187
|
| 著者 |
Ushiki T, Shigeno M, Hoshi O.
|
| タイトル |
Techniques for imaging human metaphase chromosomes in liquid conditions by atomic force microscopy.
|
| ジャーナル |
Nanotechnology
|
| Abstract |
The purpose of this study was to obtain three-dimensional images of wet chromosomes by atomic force microscopy (AFM) in liquid conditions. Human metaphase chromosomes-obtained either by chromosome spreads or by an isolation technique-were observed in a dynamic mode by AFM in a buffer solution. Under suitable operating conditions with a soft triangular cantilever (with the spring constant of 0.08-0.4 N m(-1)), clear images of fixed chromosomes in the chromosome spread were obtained by AFM. For imaging isolated chromosomes with the height of more than 400 nm, a cantilever with a high aspect ratio probing tip was required. The combination of a Q-control system and the sampling intelligent scan (SIS) system in dynamic force mode AFM was useful for obtaining high-quality images of the isolated chromosomes, in which globular or cord-like structures about 50 nm thick were clearly observed on the surface of each chromatid.
|
| 巻・号 |
19(38)
|
| ページ |
384022
|
| 公開日 |
2008-9-24
|
| DOI |
10.1088/0957-4484/19/38/384022
|
| PII |
S0957-4484(08)78059-0
|
| PMID |
21832581
|
| IF |
3.551
|
| 引用数 |
7
|
|
WOS 分野
|
PHYSICS, APPLIED
MATERIALS SCIENCE, MULTIDISCIPLINARY
NANOSCIENCE & NANOTECHNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
BALL-1(RCB0256) |
