RRC ID |
11188
|
Author |
Tsujigiwa H, Nagatsuka H, Gunduz M, Rodriguez A, Rivera RS, Legeros RZ, Inoue M, Nagai N.
|
Title |
Effects of immobilized recombinant human bone morphogenetic protein-2/succinylated type I atelocollagen on cellular activity of ST2 cells.
|
Journal |
J Biomed Mater Res A
|
Abstract |
The use of recombinant human bone morphogenetic protein-2 (rhBMP-2) to induce ectopic bone formation requires a carrier. Type I atelocollagen, a biomaterial with a porous structure, excellent operational features, and biocompatibility, is an effective carrier for rhBMP-2. However, the conventionally used lyophilized rhBMP-2/collagen mixture does not necessarily give adequate bone-induction effect. In the present study, we examined the effect of immobilizing rhBMP-2 to type I atelocollagen on the cellular activity of ST2 cells. The following results were obtained: (1) rhBMP-2 was effectively immobilized to succinylated type I atelocollagen, indicating the usefulness of succinylated type I atelocollagen in immobilization; (2) studies of alkaline phosphatase activity confirmed the effectiveness of rhBMP-2 immobilized on succinylated atelocollagen in augmenting cellular activity.
|
Volume |
75(1)
|
Pages |
210-5
|
Published |
2005-10-1
|
DOI |
10.1002/jbm.a.30416
|
PMID |
16059889
|
MeSH |
Alkaline Phosphatase / chemistry
Alkaline Phosphatase / metabolism
Animals
Biocompatible Materials / chemistry*
Blotting, Western
Bone Marrow Cells / cytology
Bone Marrow Cells / drug effects*
Bone Morphogenetic Protein 2
Bone Morphogenetic Proteins / chemistry*
Bone Morphogenetic Proteins / pharmacology
Bone and Bones / metabolism
Cell Line
Collagen / chemistry*
Collagen Type I / chemistry
Diffusion
Dose-Response Relationship, Drug
Electrophoresis, Polyacrylamide Gel
Humans
Mice
Recombinant Proteins / chemistry*
Recombinant Proteins / pharmacology
Succinates / chemistry
Time Factors
Transforming Growth Factor beta / chemistry*
Transforming Growth Factor beta / pharmacology
|
IF |
3.525
|
Times Cited |
15
|
WOS Category
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
Resource |
Human and Animal Cells |
ST2(RCB0224) |