Reference - Detail
| RRC ID | 11209 |
|---|---|
| Author | Futagawa-Saito K, Makino S, Sunaga F, Kato Y, Sakurai-Komada N, Ba-Thein W, Fukuyasu T. |
| Title | Identification of first exfoliative toxin in Staphylococcus pseudintermedius. |
| Journal | FEMS Microbiol Lett |
| Abstract |
Staphylococcus aureus, Staphylococcus hyicus, and Staphylococcus chromogenes are known to cause skin infections in human or animals by producing exfoliative toxins (ETs). Staphylococcus pseudintermedius can also cause canine pyoderma, but no exfoliative toxins or similar toxins have been reported. PCR with degenerate primers targeted to the conserved regions in ETA, ETB, and ETD from S. aureus and SHETB from S. hyicus, and subsequent chromosome walking identified a novel gene, designated as exi (exfoliative toxin of pseudintermedius) in S. pseudintermedius. EXI had significant homologies with the exfoliative toxins (43-68% identity), particularly with ETB (67.1%), ETD (67.9%), and SHETB (65.1%). Phylogenetic analysis showed close relation between EXI and ETB with a bootstrap value of 80%. Neonatal mice injected with the crude proteins from the culture supernatant or recombinant EXI showed gross blisters and/or characteristic skin exfoliation. The prevalence of exi assessed by dot-blot hybridization was 23.3% (10/43) in S. pseudintermedius isolates from canine pyoderma. The EXI reported herein is the first exfoliative toxin identified in S. pseudintermedius. |
| Volume | 301(2) |
| Pages | 176-80 |
| Published | 2009-12-1 |
| DOI | 10.1111/j.1574-6968.2009.01823.x |
| PII | FML1823 |
| PMID | 19891731 |
| MeSH | Amino Acid Sequence Animals Bacterial Toxins / genetics* Bacterial Toxins / toxicity* Cluster Analysis DNA Primers / genetics Disease Models, Animal Dog Diseases / microbiology* Dogs Exfoliatins / genetics* Exfoliatins / toxicity* Mice Molecular Sequence Data Phylogeny Poisoning Polymerase Chain Reaction / methods Pyoderma / microbiology Pyoderma / veterinary* Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid Staphylococcus / genetics* Staphylococcus / isolation & purification |
| IF | 1.987 |
| Times Cited | 30 |
| WOS Category | MICROBIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM 2422 |