| RRC ID |
11211
|
| Author |
Kongpol A, Pongtharangkul T, Kato J, Honda K, Ohtake H, Vangnai AS.
|
| Title |
Characterization of an organic-solvent-tolerant Brevibacillus agri strain 13 able to stabilize solvent/water emulsion.
|
| Journal |
FEMS Microbiol Lett
|
| Abstract |
Brevibacillus agri strain 13 was isolated and characterized as a Gram-positive organic-solvent-tolerant bacterium able to grow at 45 degrees C. It can tolerate high concentrations (5% and 20%, v/v) of various organic solvents with a broad range of log P(ow) when the organic solvent was provided as a nonaqueous layer. Although it can tolerate a number of aromatic solvents, it cannot utilize them as a sole carbon source. The surface characteristics of cells exposed to organic solvent were investigated using the bacterial adhesion to hydrocarbon test, a contact angle measurement, zeta potential determination, and fluorescence microscopy analysis and compared with that of nonexposed cells. The results showed that although it has a hydrophilic cell surface, it has a unique indigenous cell surface characteristic in which the cells can stabilize solvent-in-water emulsion by adhering to the solvent-water interface of the solvent droplets. The tolerance and predilection of B. agri strain 13 toward organic solvents may suggest its potential application as a whole-cell biocatalyst for the biotransformation process of water-immiscible substrate(s).
|
| Volume |
297(2)
|
| Pages |
225-33
|
| Published |
2009-8-1
|
| DOI |
10.1111/j.1574-6968.2009.01684.x
|
| PII |
FML1684
|
| PMID |
19548892
|
| MeSH |
Emulsions / chemistry
Emulsions / metabolism
Gram-Positive Bacteria / chemistry*
Gram-Positive Bacteria / metabolism
Organic Chemicals / chemistry
Organic Chemicals / metabolism*
Solvents / chemistry
Solvents / metabolism*
Surface Properties
Water / chemistry
Water / metabolism*
|
| IF |
1.987
|
| Times Cited |
20
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM 9067 |
