RRC ID 11216
著者 Kenzaka T, Ishidoshiro A, Tani K, Nasu M.
タイトル Scanning electron microscope imaging of bacteria based on DNA sequence.
ジャーナル Lett Appl Microbiol
Abstract AIMS:To develop a scanning electron microscopic approach using in situ hybridization (SEM-ISH) for gaining both genetic and morphological information about target bacteria.
METHODS AND RESULTS:Target cells were hybridized with DNA-targeted polynucleotide probes, and a tyramide signal amplification system was used to increase the sensitivity. The protocol of SEM-ISH enabled to detect low copy number target DNA sequences in individual cells.
CONCLUSIONS:SEM-ISH allowed the in situ detection of bacteria carrying a specific gene.
SIGNIFICANCE AND IMPACT OF THE STUDY:Combining morphological study with SEM and ISH techniques appears to be a valuable tool to understand the spatial distribution of target cells in complex microbial communities on various materials.
巻・号 49(6)
ページ 796-9
公開日 2009-12-1
DOI 10.1111/j.1472-765X.2009.02680.x
PII LAM2680
PMID 19889108
MeSH Bacteria / genetics Bacteria / isolation & purification* Bacteria / ultrastructure DNA Probes DNA, Bacterial / analysis Microscopy, Electron, Scanning / methods* Nucleic Acid Amplification Techniques Nucleic Acid Hybridization
IF 2.173
引用数 3
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
一般微生物 JCM 1234