Reference - Detail
| RRC ID | 11217 |
|---|---|
| Author | O'Connell Motherway M, O'Driscoll J, Fitzgerald GF, Van Sinderen D. |
| Title | Overcoming the restriction barrier to plasmid transformation and targeted mutagenesis in Bifidobacterium breve UCC2003. |
| Journal | Microb Biotechnol |
| Abstract |
In silico analysis of the Bifidobacterium breve UCC2003 genome predicted two distinct loci, which encode three different restriction/modification systems, each comprising a modification methylase and a restriction endonuclease. Based on sequence homology and observed protection against restriction we conclude that the first restriction endonuclease, designated BbrI, is an isoschizomer of BbeI, the second, BbrII, is a neoschizomer of SalI, while the third, BbrIII, is an isoschizomer of PstI. Expression of each of the B. breve UCC2003 methylase-encoding genes in B. breve JCM 7017 established that BbrII and BbrIII are active and restrict incoming DNA. By exploiting knowledge on restriction/modification in B. breve UCC2003 we successfully increased the transformation efficiency to a level that allows the reliable generation of mutants by homologous recombination using a non-replicative plasmid. |
| Volume | 2(3) |
| Pages | 321-32 |
| Published | 2009-5-1 |
| DOI | 10.1111/j.1751-7915.2008.00071.x |
| PMID | 21261927 |
| PMC | PMC3815753 |
| MeSH | Amino Acid Sequence Bacterial Proteins / chemistry Bacterial Proteins / genetics* Bacterial Proteins / metabolism Bifidobacterium / chemistry Bifidobacterium / enzymology* Bifidobacterium / genetics* DNA Restriction Enzymes / chemistry DNA Restriction Enzymes / genetics* DNA Restriction Enzymes / metabolism Molecular Sequence Data Mutagenesis* Plasmids / genetics* Sequence Alignment Transformation, Bacterial* |
| IF | 5.328 |
| Times Cited | 73 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 43 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM 7017 JCM 7019 |