| RRC ID |
11218
|
| Author |
Locascio RG, Niñonuevo MR, Kronewitter SR, Freeman SL, German JB, Lebrilla CB, Mills DA.
|
| Title |
A versatile and scalable strategy for glycoprofiling bifidobacterial consumption of human milk oligosaccharides.
|
| Journal |
Microb Biotechnol
|
| Abstract |
Human milk contains approximately 200 complex oligosaccharides believed to stimulate the growth and establishment of a protective microbiota in the infant gut. The lack of scalable analytical techniques has hindered the measurement of bacterial metabolism of these and other complex prebiotic oligosaccharides. An in vitro, multi-strain, assay capable of measuring kinetics of bacterial growth and detailed oligosaccharide consumption analysis by FTICR-MS was developed and tested simultaneously on 12 bifidobacterial strains. For quantitative consumption, deuterated and reduced human milk oligosaccharide (HMO) standards were used. A custom software suite developed in house called Glycolyzer was used to process the large amounts of oligosaccharide mass spectra automatically with (13)C corrections based on de-isotoping protocols. High growth on HMOs was characteristic of Bifidobacterium longum biovar infantis strains, which consumed nearly all available substrates, while other bifidobacterial strains tested, B. longum bv. longum, B. adolescentis, B. breve and B. bifidum, showed low or only moderate growth ability. Total oligosaccharide consumption ranged from a high of 87% for B. infantis JCM 7009 to only 12% for B. adolescentis ATCC 15703. A detailed analysis of consumption glycoprofiles indicated strain-specific capabilities towards differential metabolism of milk oligosaccharides. This method overcomes previous limitations in the quantitative, multi-strain analysis of bacterial metabolism of HMOs and represents a novel approach towards understanding bacterial consumption of complex prebiotic oligosaccharides.
|
| Volume |
2(3)
|
| Pages |
333-42
|
| Published |
2009-5-1
|
| DOI |
10.1111/j.1751-7915.2008.00072.x
|
| PMID |
21261928
|
| PMC |
PMC3815754
|
| MeSH |
Bifidobacterium / chemistry
Bifidobacterium / growth & development
Bifidobacterium / metabolism*
Gastrointestinal Tract / metabolism
Gastrointestinal Tract / microbiology
Humans
Milk, Human / chemistry
Milk, Human / metabolism*
Models, Biological
Oligosaccharides / chemistry
Oligosaccharides / metabolism*
Software
|
| IF |
5.328
|
| Times Cited |
80
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM 7009
JCM 7010
JCM 7007
JCM 7011
JCM 1260
JCM 1254
JCM 11347
JCM 1255 |
