RRC ID 1124
Author Makimura K, Oguri T, Mikami Y, Kume H, Hanazawa R, Abe M, Ikeda R, Shinoda T.
Title Multicenter evaluation of commercial frozen plates for microdilution broth antifungal susceptibility testing of yeasts and comparison of MIC limits recommended in NCCLS M27-A2.
Journal Microbiol. Immunol.
Abstract A commercial kit, Frozen Plate for Antifungal Susceptibility Testing of Yeasts, Eiken (Eiken Chemical Co., Ltd., Tokyo), was tested in a multi-institute study to evaluate the agreement between interinstitute MICs and National Committee for Clinical Laboratory Standards (NCCLS) M27-A2 recommendation limits of MIC value. The kit was reported as a method equivalent to the standardized guidelines for antifungal susceptibility testing by the Committee for Clinical Laboratory Standards-1994, the Japanese Society for Medical Mycology, and which is widely used in Japan for amphotericin B, flucytosine, fluconazole, miconazole, and itraconazole. The degrees of inter-institute and NCCLS agreements were good to excellent especially with 48-hr incubation for all antifungal agents. However, the percent agreements to NCCLS recommendations against itraconazole were poor. Overall, MIC values obtained using the frozen plate antifungal susceptibility testing kit, with 48-hr incubation, were thought to be reliable and convenient alternatives to the data obtained by the NCCLS M27-A2 reference macrodilution and microdilution method. This kit will allow matching of results between international laboratories. However, the MIC value for itraconazole requires careful interpretation.
Volume 49(2)
Pages 97-106
Published 2005
PII JST.JSTAGE/mandi/49.97
PMID 15722594
MeSH Antifungal Agents / pharmacology Culture Media Freezing Japan Microbial Sensitivity Tests / methods* Microbial Sensitivity Tests / standards Reagent Kits, Diagnostic* / standards Yeasts / drug effects Yeasts / growth & development
IF 1.335
Times Cited 4
Pathogenic microorganisms IFM 5446 IFM 5462 IFM 5774 IFM 5776 IFM 5777 IFM 5801 IFM 40205 IFM 40213 IFM 40218 IFM 46066 IFM 46829 IFM 46834