| RRC ID |
11250
|
| 著者 |
Nakanishi T, Goto C, Kobayashi M, Kang W, Suzuki T, Dohmae N, Matsumoto S, Shimada T, Katsuma S.
|
| タイトル |
Comparative studies of lepidopteran baculovirus-specific protein FP25K: development of a novel Bombyx mori nucleopolyhedrovirus-based vector with a modified fp25K gene.
|
| ジャーナル |
J Virol
|
| Abstract |
Lepidopteran baculovirus-specific protein FP25K performs many roles during the infection cycle, including functions in the production of occlusion bodies (OBs) and budded viruses (BVs), oral infection, and postmortem host degradation. To explore the common and specific functions of FP25K proteins among lepidopteran baculoviruses, we performed comparative analyses of FP25K proteins from group I and group II nucleopolyhedroviruses (NPVs) and granulovirus (GV). Using recombinant Bombyx mori NPVs (BmNPVs), we showed that the FP25Ks from NPVs were able to eliminate all the phenotypic defects observed in an infection with a BmNPV mutant lacking functional fp25K but that FP25K from GV did not show abilities to recover oral infectivity and postmortem host degradation. We also observed that introduction of Autographa californica multiple NPV (AcMNPV) fp25K into the BmNPV genome enhanced OB and BV production. According to these results, we generated a novel BmNPV-based expression vector with AcMNPV fp25K and examined its potential in BmN cells and B. mori larvae. Our results showed that the introduction of AcMNPV fp25K significantly increases the expression of foreign gene products in cultured cells and shortens the time for obtaining the secreted recombinant proteins from larval hemolymph.
|
| 巻・号 |
84(10)
|
| ページ |
5191-200
|
| 公開日 |
2010-5-1
|
| DOI |
10.1128/JVI.00099-10
|
| PII |
JVI.00099-10
|
| PMID |
20219904
|
| PMC |
PMC2863835
|
| MeSH |
Animals
Biotechnology / methods
Gene Knockout Techniques
Genetic Complementation Test
Genetic Vectors*
Granulovirus / genetics
Granulovirus / physiology*
Lepidoptera / virology*
Nucleocapsid Proteins / genetics
Nucleocapsid Proteins / physiology*
Nucleopolyhedroviruses / genetics
Nucleopolyhedroviruses / physiology*
Recombinant Proteins / biosynthesis
|
| IF |
4.501
|
| 引用数 |
17
|
|
WOS 分野
|
VIROLOGY
|
| リソース情報 |
| カイコ |
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