| RRC ID |
11252
|
| 著者 |
Meng Y, Katsuma S, Mita K, Shimada T.
|
| タイトル |
Abnormal red body coloration of the silkworm, Bombyx mori, is caused by a mutation in a novel kynureninase.
|
| ジャーナル |
Genes Cells
|
| Abstract |
Larvae of the body color mutant red blood (rb) of the silkworm, Bombyx mori, display reddish skin whose hemolymph becomes red in air, whereas hemolymphs of normal strains become black during melanization. The irregular coloring was assumed to result from an abnormal accumulation of 3-hydroxykynurenine. However, the gene responsible for the rb phenotype is not yet known. Here, we provide evidence that the rb gene corresponds to a novel bacterial-type kynureninase gene, BmKynu. Kynureninase (KYNU) hydrolyzes kynurenine and 3-hydroxykynurenine to anthranilic acid and 3-hydroxyanthranilic acid, respectively. KYNU has been identified in microorganisms and animals but not in insects. Therefore, BmKynu is the first KYNU gene observed in insects. Our results clearly showed that a point mutation (T102I) in BmKYNU of the rb strain led to a marked decrease in KYNU activity, presumably resulting in abnormal accumulation of 3-hydroxykynurenine. Additionally, linkage analysis indicated that no recombination between rb and BmKynu was detected. We conclude that T102I in BmKYNU causes the red body coloration in the rb strain. Our study proves that B. mori has a unique side branch in the kynurenine pathway, distinctly different from other insects.
|
| 巻・号 |
14(2)
|
| ページ |
129-40
|
| 公開日 |
2009-2-1
|
| DOI |
10.1111/j.1365-2443.2008.01257.x
|
| PII |
GTC1257
|
| 解説 |
rb(赤血）の原因遺伝子はBmKynuである。BmKynuはキヌレニナーゼをコードする。k20 系統(rb/rb)のBmKynuでは、酵素活性を低下させる点突然変異が存在する。
|
| PMID |
19170761
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
Bombyx / enzymology
Bombyx / genetics*
Bombyx / physiology*
Cloning, Molecular
Color*
DNA Mutational Analysis
Gene Expression Regulation, Enzymologic
Genetic Linkage
Hydrolases / genetics*
Hydrolases / isolation & purification
Hydrolases / metabolism
Hydrolases / physiology
Models, Biological
Molecular Sequence Data
Mutation / physiology
Phylogeny
Polymorphism, Single Nucleotide / physiology
Sequence Homology, Amino Acid
Skin Pigmentation / genetics*
|
| IF |
1.655
|
| 引用数 |
25
|
|
WOS 分野
|
GENETICS & HEREDITY
CELL BIOLOGY
|
| リソース情報 |
| カイコ |
p50
e36
l70
a65
i41
k20 |
