Reference - Detail
| RRC ID | 11257 |
|---|---|
| Author | Orihara Y, Hamamoto H, Kasuga H, Shimada T, Kawaguchi Y, Sekimizu K. |
| Title | A silkworm baculovirus model for assessing the therapeutic effects of antiviral compounds: characterization and application to the isolation of antivirals from traditional medicines. |
| Journal | J Gen Virol |
| Abstract |
Ganciclovir, foscarnet, vidarabine and ribavirin, which are used to treat viral infections in humans, inhibited the proliferation of a baculovirus (Bombyx mori nucleopolyhedrovirus) in BmN4 cells, a cultured silkworm cell line. These antiviral agents inhibited the proliferation of baculovirus in silkworm body fluid and had therapeutic effects. Using the silkworm infection model, the antiviral activity of Kampo medicines was screened and it was found that cinnamon bark, a component of the traditional Japanese medicine Mao-to, had a therapeutic effect. Based on the therapeutic activity, the antiviral substance was purified. Nuclear magnetic resonance analysis of the purified fraction revealed that the antiviral activity was due to cinnzeylanine, which has previously been isolated from Cinnamomum zeylanicum. Cinnzeylanine inhibits the proliferation of herpes simplex virus type 1 in Vero cells. These results suggest that the silkworm-baculovirus infection model is useful for screening antiviral agents that are effective for treating humans infected with DNA viruses. |
| Volume | 89(Pt 1) |
| Pages | 188-194 |
| Published | 2008-1-1 |
| DOI | 10.1099/vir.0.83208-0 |
| PMID | 18089742 |
| MeSH | Animals Antiviral Agents / therapeutic use* Baculoviridae / drug effects* Baculoviridae / growth & development* Baculoviridae / isolation & purification Bombyx / virology* Cinnamomum zeylanicum Female Humans Japan Ovum / virology Plant Extracts / therapeutic use* Viral Plaque Assay |
| IF | 3.376 |
| Times Cited | 35 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY VIROLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Silkworms | |