| RRC ID |
11259
|
| Author |
Wen H, Lan X, Cheng T, He N, Shiomi K, Kajiura Z, Zhou Z, Xia Q, Xiang Z, Nakagaki M.
|
| Title |
Sequence structure and expression pattern of a novel anionic defensin-like gene from silkworm (Bombyx mori).
|
| Journal |
Mol Biol Rep
|
| Abstract |
A defensin-like gene, BmdefA, was rediscovered in the silkworm genome and expressed sequence tags databases. The open reading frame of BmdefA encodes a prepropeptide consisting of a 22-residue signal peptide, a 34-residue propeptide, and a 36-residue mature peptide with a molecular mass of 4.0 kDa. The mature peptide possesses the characteristic six-cysteine motif of insect defensins, and its predicted isoelectric point is 4.12, indicating it is a novel anionic defensin. An intron is present in BmdefA and several cis-regulatory elements are in the regulating region. It is transcribed constitutively at a high level in the hemocyte, silk gland, head, and ovary of the silkworm larvae, and in the fat body of early-stage pupae and moth. BmdefA is also strongly induced by immune challenge. These results suggest that BmdefA plays an important role in both immunity and metamorphosis.
|
| Volume |
36(4)
|
| Pages |
711-6
|
| Published |
2009-4-1
|
| DOI |
10.1007/s11033-008-9233-4
|
| PMID |
18360778
|
| MeSH |
Amino Acid Sequence
Animals
Anions / chemistry
Base Sequence
Bombyx / chemistry*
Bombyx / genetics
Bombyx / metabolism*
Conserved Sequence
Defensins / chemistry*
Defensins / classification
Defensins / genetics
Defensins / metabolism*
Gene Expression Regulation / genetics*
Genome, Insect / genetics
Molecular Sequence Data
Sequence Alignment
Transcription, Genetic / genetics
|
| IF |
1.402
|
| Times Cited |
32
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Silkworms |
|
