| RRC ID |
11266
|
| Author |
Tsubota T, Shimomura M, Ogura T, Seino A, Nakakura T, Mita K, Shinoda T, Shiotsuki T.
|
| Title |
Molecular characterization and functional analysis of novel carboxyl/cholinesterases with GQSAG motif in the silkworm Bombyx mori.
|
| Journal |
Insect Biochem Mol Biol
|
| Abstract |
We have previously cloned and characterized BmJHE, a juvenile hormone (JH)-selective esterase (JHE) that is important for JH titer regulation in the silkworm Bombyx mori. Here, we sought to determine whether multiple genes might function as JH-specific esterase in this species. We searched for putative carboxyl/cholinesterase (CCE) genes having GQSAG, a highly conserved motif in JHE, by the use of silkworm genomic database. Five novel CCE genes (Bmcce-1-5) were identified and their cDNA sequences and intron-exon structures were determined. We investigated the developmental expression patterns of these CCE genes by real-time quantitative PCR analysis and found that their expression patterns varied among developmental stages and organs. Of the proteins produced by the five genes, only BmCCE-5 had the ability to degrade JH; however, this protein might not function as a JH-specific esterase in vivo as it had a high K(m) value for JH. On the other hand, BmCCE-5 degraded general esterase substrates efficiently. Since Bmcce-5 was strongly expressed in Malpighian tubules and the gut, it might function in digestion or xenobiotic metabolism. Our results suggest that of the CCEs containing a GQSAG motif only BmJHE can function as a JH-specific degradation enzyme in the silkworm.
|
| Volume |
40(2)
|
| Pages |
100-12
|
| Published |
2010-2-1
|
| DOI |
10.1016/j.ibmb.2009.12.015
|
| PII |
S0965-1748(09)00195-7
|
| PMID |
20060470
|
| MeSH |
Amino Acid Motifs*
Amino Acid Sequence
Animals
Base Sequence
Bombyx / enzymology*
Carboxylic Ester Hydrolases / chemistry
Carboxylic Ester Hydrolases / genetics*
Carboxylic Ester Hydrolases / metabolism
Cholinesterases / chemistry
Cholinesterases / genetics*
Cholinesterases / metabolism
Cloning, Molecular
DNA Primers
Exons
Introns
Kinetics
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
|
| IF |
3.827
|
| Times Cited |
28
|
|
WOS Category
|
ENTOMOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Silkworms |
NA |
