論文 - 詳細
| RRC ID | 11277 |
|---|---|
| 著者 | Mon H, Sugahara R, Hong SM, Lee JM, Kamachi Y, Kawaguchi Y, Kusakabe T. |
| タイトル | Analysis of protein interactions with two-hybrid system in cultured insect cells. |
| ジャーナル | Anal Biochem |
| Abstract |
Many biological processes are usually coupled to the formation of protein complexes. The yeast two-hybrid system is a powerful tool for analyzing protein-protein interactions. Different patterns of protein modifications, such as glycosylation, phosphorylation, and acetylation, may affect the ability of proteins to interact. In this study, we developed the two-hybrid system that can be used in insect cells. To validate the insect two-hybrid (I2H) system, we analyzed and confirmed the known oligomer or dimer formation of silkworm Rad51 or RPA2-RPA3, respectively. The results established the feasibility of the I2H system for efficient analysis of protein interaction under conditions that closely reflect the normal physiological environment. |
| 巻・号 | 392(2) |
| ページ | 180-2 |
| 公開日 | 2009-9-15 |
| DOI | 10.1016/j.ab.2009.05.033 |
| PII | S0003-2697(09)00367-4 |
| PMID | 19481053 |
| MeSH | Animals Bombyx / genetics Bombyx / metabolism* Cell Line Insect Proteins / analysis* Insect Proteins / genetics Insect Proteins / metabolism Protein Binding Protein Subunits / analysis Protein Subunits / genetics Protein Subunits / metabolism Rad51 Recombinase / analysis* Rad51 Recombinase / genetics Rad51 Recombinase / metabolism Replication Protein A / analysis* Replication Protein A / genetics Replication Protein A / metabolism Two-Hybrid System Techniques* |
| IF | 2.877 |
| 引用数 | 16 |
| WOS 分野 | CHEMISTRY, ANALYTICAL BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY |
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