| RRC ID |
11290
|
| Author |
Moribe Y, Oka K, Niimi T, Yamashita O, Yaginuma T.
|
| Title |
Expression of heat shock protein 70a mRNA in Bombyx mori diapause eggs.
|
| Journal |
J Insect Physiol
|
| Abstract |
In an effort to understand whether heat shock protein 70 (Hsp70) participates in the environmental 5 degrees C signal reception/transduction toward breaking embryonic diapause of the silkworm Bombyx mori, we isolated a cDNA for Hsp70a and examined the expression of Hsp70a mRNA in B. mori diapause and nondiapause eggs by quantitative real-time PCR. Hsp70a mRNA gradually increased in diapause eggs continuously kept at 25 degrees C after oviposition to maintain diapause. When diapause eggs were exposed to the diapause-terminating condition of 5 degrees C beginning at 2 days post-oviposition, Hsp70a mRNA increased beginning at 5 days post-cold treatment. Even in nondiapause eggs, Hsp70a mRNA increased slightly with exposure to 5 degrees C. These results suggest that Hsp70a is involved in reception/transduction of the diapause-terminating (5 degrees C) signal via gene activation. The expression patterns of Hsp70a mRNA are discussed in relation to those of the cold-response gene Samui.
|
| Volume |
56(9)
|
| Pages |
1246-52
|
| Published |
2010-9-1
|
| DOI |
10.1016/j.jinsphys.2010.03.023
|
| PII |
S0022-1910(10)00098-3
|
| PMID |
20371249
|
| MeSH |
Acclimatization / physiology*
Amino Acid Sequence
Animals
Bombyx / genetics
Bombyx / metabolism*
Bombyx / physiology
DNA Primers / genetics
DNA, Complementary / genetics
Gene Expression Regulation / physiology*
HSP70 Heat-Shock Proteins / genetics
HSP70 Heat-Shock Proteins / metabolism*
Molecular Sequence Data
Ovum / metabolism*
RNA, Messenger / metabolism*
Sequence Alignment
Sequence Analysis, DNA
Temperature
|
| IF |
2.246
|
| Times Cited |
15
|
|
WOS Category
|
ENTOMOLOGY
ZOOLOGY
PHYSIOLOGY
|
| Resource |
| Silkworms |
|
