RRC ID 11303
著者 Ohtsuka K, Atsumi T, Fukushima Y, Shiomi K.
タイトル Identification of a cis-regulatory element that directs prothoracicotropic hormone gene expression in the silkworm Bombyx mori.
ジャーナル Insect Biochem Mol Biol
Abstract In the silkworm Bombyx mori and other insects, prothoracicotropic hormone (PTTH) plays a central role in controlling molting and metamorphosis by stimulating the prothoracic glands to synthesize and release the molting hormone ecdysone. Using an AcNPV (Autographa californica nucleopolyhedrovirus)-mediated transient gene transfer system, we identified a cis-regulatory element that participates in the decision to switch expression of PTTH on or off in PTTH-producing neurosecretory cells (PTPCs). The nucleotide sequence of this cis-regulatory element is similar to a cis-regulatory element that participates in direction of expression of diapause hormone-pheromone biosynthesis activating neuropeptide gene (DH-PBAN) (Shiomi et al., 2007). Furthermore, we found that B. mori Pitx (BmPitx), a bicoid-like homeobox transcription factor, binds the element and activates PTTH expression. Therefore, we propose that the cell-specific expression of two neuropeptide hormone genes, PTTH and DH-PBAN, is activated by the Pitx transcription factor, which may act as a pan-activator in the insect neuroendocrine system and in vertebrate pituitary cells.
巻・号 41(6)
ページ 356-61
公開日 2011-6-1
DOI 10.1016/j.ibmb.2011.02.002
PII S0965-1748(11)00040-3
PMID 21324358
MeSH Amino Acid Sequence Animals Base Sequence Bombyx / genetics Bombyx / metabolism* Cloning, Molecular Gene Expression Regulation* Gene Transfer Techniques Homeodomain Proteins / genetics Homeodomain Proteins / metabolism* Insect Hormones* / biosynthesis Insect Hormones* / genetics Insect Proteins / genetics Insect Proteins / metabolism* Larva / genetics Larva / metabolism Microinjections Molecular Sequence Data Molting / genetics Neuropeptides / genetics Neuropeptides / metabolism* Neurosecretory Systems / metabolism Nucleopolyhedroviruses Protein Precursors / genetics Protein Precursors / metabolism* Pupa / genetics Pupa / metabolism Transcription Factors / genetics Transcription Factors / metabolism
IF 3.827
引用数 2
WOS 分野 ENTOMOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
カイコ NA