論文 - 詳細
RRC ID | 11303 |
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著者 | Ohtsuka K, Atsumi T, Fukushima Y, Shiomi K. |
タイトル | Identification of a cis-regulatory element that directs prothoracicotropic hormone gene expression in the silkworm Bombyx mori. |
ジャーナル | Insect Biochem Mol Biol |
Abstract |
In the silkworm Bombyx mori and other insects, prothoracicotropic hormone (PTTH) plays a central role in controlling molting and metamorphosis by stimulating the prothoracic glands to synthesize and release the molting hormone ecdysone. Using an AcNPV (Autographa californica nucleopolyhedrovirus)-mediated transient gene transfer system, we identified a cis-regulatory element that participates in the decision to switch expression of PTTH on or off in PTTH-producing neurosecretory cells (PTPCs). The nucleotide sequence of this cis-regulatory element is similar to a cis-regulatory element that participates in direction of expression of diapause hormone-pheromone biosynthesis activating neuropeptide gene (DH-PBAN) (Shiomi et al., 2007). Furthermore, we found that B. mori Pitx (BmPitx), a bicoid-like homeobox transcription factor, binds the element and activates PTTH expression. Therefore, we propose that the cell-specific expression of two neuropeptide hormone genes, PTTH and DH-PBAN, is activated by the Pitx transcription factor, which may act as a pan-activator in the insect neuroendocrine system and in vertebrate pituitary cells. |
巻・号 | 41(6) |
ページ | 356-61 |
公開日 | 2011-6-1 |
DOI | 10.1016/j.ibmb.2011.02.002 |
PII | S0965-1748(11)00040-3 |
PMID | 21324358 |
MeSH | Amino Acid Sequence Animals Base Sequence Bombyx / genetics Bombyx / metabolism* Cloning, Molecular Gene Expression Regulation* Gene Transfer Techniques Homeodomain Proteins / genetics Homeodomain Proteins / metabolism* Insect Hormones* / biosynthesis Insect Hormones* / genetics Insect Proteins / genetics Insect Proteins / metabolism* Larva / genetics Larva / metabolism Microinjections Molecular Sequence Data Molting / genetics Neuropeptides / genetics Neuropeptides / metabolism* Neurosecretory Systems / metabolism Nucleopolyhedroviruses Protein Precursors / genetics Protein Precursors / metabolism* Pupa / genetics Pupa / metabolism Transcription Factors / genetics Transcription Factors / metabolism |
IF | 3.827 |
引用数 | 2 |
WOS 分野 | ENTOMOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
リソース情報 | |
カイコ | NA |