| RRC ID |
1131
|
| 著者 |
Chibana H, Oka N, Nakayama H, Aoyama T, Magee BB, Magee PT, Mikami Y.
|
| タイトル |
Sequence finishing and gene mapping for Candida albicans chromosome 7 and syntenic analysis against the Saccharomyces cerevisiae genome.
|
| ジャーナル |
Genetics
|
| Abstract |
The size of the genome in the opportunistic fungus Candida albicans is 15.6 Mb. Whole-genome shotgun sequencing was carried out at Stanford University where the sequences were assembled into 412 contigs. C. albicans is a diploid basically, and analysis of the sequence is complicated due to repeated sequences and to sequence polymorphism between homologous chromosomes. Chromosome 7 is 1 Mb in size and the best characterized of the 8 chromosomes in C. albicans. We assigned 16 of the contigs, ranging in length from 7309 to 267,590 bp, to chromosome 7 and determined sequences of 16 regions. These regions included four gaps, a misassembled sequence, and two major repeat sequences (MRS) of >16 kb. The length of the continuous sequence attained was 949,626 bp and provided complete coverage of chromosome 7 except for telomeric regions. Sequence analysis was carried out and predicted 404 genes, 11 of which included at least one intron. A 7-kb indel, which might be caused by a retrotransposon, was identified as the largest difference between the homologous chromosomes. Synteny analysis revealed that the degree of synteny between C. albicans and Saccharomyces cerevisiae is too weak to use for completion of the genomic sequence in C. albicans.
|
| 巻・号 |
170(4)
|
| ページ |
1525-37
|
| 公開日 |
2005-8-1
|
| DOI |
10.1534/genetics.104.034652
|
| PII |
genetics.104.034652
|
| PMID |
15937140
|
| PMC |
PMC1449773
|
| MeSH |
Amino Acid Sequence
Base Pairing
Base Sequence
Candida albicans / genetics*
Chromosomes, Fungal*
DNA, Fungal
Genetic Linkage
Genome, Fungal*
Introns
Molecular Sequence Data
Nucleic Acid Amplification Techniques
Open Reading Frames
Physical Chromosome Mapping*
Saccharomyces cerevisiae / genetics*
Sequence Analysis, DNA
Synteny*
|
| IF |
4.015
|
| 引用数 |
16
|
|
WOS 分野
|
GENETICS & HEREDITY
|
| リソース情報 |
| 病原微生物 |
C. albicans SC5314? |
