RRC ID 1131
Author Chibana H, Oka N, Nakayama H, Aoyama T, Magee BB, Magee PT, Mikami Y.
Title Sequence finishing and gene mapping for Candida albicans chromosome 7 and syntenic analysis against the Saccharomyces cerevisiae genome.
Journal Genetics
Abstract The size of the genome in the opportunistic fungus Candida albicans is 15.6 Mb. Whole-genome shotgun sequencing was carried out at Stanford University where the sequences were assembled into 412 contigs. C. albicans is a diploid basically, and analysis of the sequence is complicated due to repeated sequences and to sequence polymorphism between homologous chromosomes. Chromosome 7 is 1 Mb in size and the best characterized of the 8 chromosomes in C. albicans. We assigned 16 of the contigs, ranging in length from 7309 to 267,590 bp, to chromosome 7 and determined sequences of 16 regions. These regions included four gaps, a misassembled sequence, and two major repeat sequences (MRS) of >16 kb. The length of the continuous sequence attained was 949,626 bp and provided complete coverage of chromosome 7 except for telomeric regions. Sequence analysis was carried out and predicted 404 genes, 11 of which included at least one intron. A 7-kb indel, which might be caused by a retrotransposon, was identified as the largest difference between the homologous chromosomes. Synteny analysis revealed that the degree of synteny between C. albicans and Saccharomyces cerevisiae is too weak to use for completion of the genomic sequence in C. albicans.
Volume 170(4)
Pages 1525-37
Published 2005-8
DOI 10.1534/genetics.104.034652
PII genetics.104.034652
PMID 15937140
PMC PMC1449773
MeSH Amino Acid Sequence Base Pairing Base Sequence Candida albicans / genetics* Chromosomes, Fungal* DNA, Fungal Genetic Linkage Genome, Fungal* Introns Molecular Sequence Data Nucleic Acid Amplification Techniques Open Reading Frames Physical Chromosome Mapping* Saccharomyces cerevisiae / genetics* Sequence Analysis, DNA Synteny*
IF 3.564
Times Cited 14
Pathogenic microorganisms C. albicans SC5314?