| Abstract |
To understand the inhibitory effects of high temperature on insect growth at the cellular level, we investigated the influence of high temperature on the proliferation and division of larval hemocytes in the silkworm, Bombyx mori. Although the total number of hemocytes in the larval body increased enormously over time at 26 degrees C, no increase was observed at 38 degrees C. The number of mitotic hemocytes in circulation increased between days 1 and 2 of the fourth larval stage at 26 degrees C, whereas fewer hemocytes were observed at 38 degrees C. Laser scanning cytometry revealed that the DNA content of hemocytes collected from the fourth-stadium larvae was predominantly 2C, 4C, and 8C, and the proportion of each type of hemocyte changed dynamically with development during the fourth instar. Specifically, the proportion of hemocytes with a higher DNA content increased gradually during the feeding phase then decreased during the molting phase at 26 degrees C; in contrast, no decrease was observed at 38 degrees C. The heat-induced accumulation of 8C hemocytes was mainly detected in granulocytes and plasmatocytes. These data suggest that high temperatures induce a G(2) arrest in larval hemocytes.
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