RRC ID 11333
Author Hong SM, Kusakabe T, Lee JM, Tatsuke T, Kawaguchi Y, Kang MW, Kang SW, Kim KA, Nho SK.
Title Structure and expression analysis of the cecropin-E gene from the silkworm, Bombyx mori.
Journal Biosci Biotechnol Biochem
Abstract Cecropins belong to the antibacterial peptides family and are induced after injection of bacteria or their cell-wall components. By silkworm cDNA microarray analysis, a novel type of Cecropin family gene was identified as a cDNA up-regulated in early embryo, 1 day after oviposition. The cDNA isolated was 394 bp with 198 ORF translating 65 amino acids, encoding BmCecropin-E (BmCec-E). Using Southern hybridization and genome search analysis, the number of BmCec-E gene was estimated to be at least two per haploid, which consisted of two exons, as in other Cecropin family members. BmCec-E mRNA was expressed transiently 1 day after egg-laying (AEL, germ-band formation stage), and was specifically expressed in the degenerating intestine during the pre-pupal and pupal stages, unlike other Cecropin family genes. Immune challenge analysis showed that BmCec-E gene expression was more strongly induced by Escherichia coli (gram-negative) than by Micrococus luteus (gram-positive), and not by virus injection. By bacterial challenge, expression of BmCec-E mRNA was induced 12 h after injection, and was maintained for 24 h. Expression of BmCec-E after immune challenge was observed strongly in excretory organs, such as hindgut and malphigian, slightly in fat body, skin, and midgut.
Volume 72(8)
Pages 1992-8
Published 2008-8-1
DOI 10.1271/bbb.80082
PII JST.JSTAGE/bbb/80082
PMID 18685215
MeSH Amino Acid Sequence Animals Base Sequence Bombyx / chemistry Bombyx / embryology Bombyx / growth & development Bombyx / metabolism* Cecropins / chemistry* Cecropins / genetics Cecropins / immunology Cecropins / metabolism* Cloning, Molecular Gene Expression Regulation, Developmental Genome / genetics Insect Proteins Molecular Sequence Data Phylogeny Sequence Alignment Sequence Homology, Amino Acid
IF 1.516
Times Cited 16
WOS Category FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Silkworms ?