論文 - 詳細
| RRC ID | 11333 |
|---|---|
| 著者 | Hong SM, Kusakabe T, Lee JM, Tatsuke T, Kawaguchi Y, Kang MW, Kang SW, Kim KA, Nho SK. |
| タイトル | Structure and expression analysis of the cecropin-E gene from the silkworm, Bombyx mori. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
Cecropins belong to the antibacterial peptides family and are induced after injection of bacteria or their cell-wall components. By silkworm cDNA microarray analysis, a novel type of Cecropin family gene was identified as a cDNA up-regulated in early embryo, 1 day after oviposition. The cDNA isolated was 394 bp with 198 ORF translating 65 amino acids, encoding BmCecropin-E (BmCec-E). Using Southern hybridization and genome search analysis, the number of BmCec-E gene was estimated to be at least two per haploid, which consisted of two exons, as in other Cecropin family members. BmCec-E mRNA was expressed transiently 1 day after egg-laying (AEL, germ-band formation stage), and was specifically expressed in the degenerating intestine during the pre-pupal and pupal stages, unlike other Cecropin family genes. Immune challenge analysis showed that BmCec-E gene expression was more strongly induced by Escherichia coli (gram-negative) than by Micrococus luteus (gram-positive), and not by virus injection. By bacterial challenge, expression of BmCec-E mRNA was induced 12 h after injection, and was maintained for 24 h. Expression of BmCec-E after immune challenge was observed strongly in excretory organs, such as hindgut and malphigian, slightly in fat body, skin, and midgut. |
| 巻・号 | 72(8) |
| ページ | 1992-8 |
| 公開日 | 2008-8-1 |
| DOI | 10.1271/bbb.80082 |
| PII | JST.JSTAGE/bbb/80082 |
| PMID | 18685215 |
| MeSH | Amino Acid Sequence Animals Base Sequence Bombyx / chemistry Bombyx / embryology Bombyx / growth & development Bombyx / metabolism* Cecropins / chemistry* Cecropins / genetics Cecropins / immunology Cecropins / metabolism* Cloning, Molecular Gene Expression Regulation, Developmental Genome / genetics Insect Proteins Molecular Sequence Data Phylogeny Sequence Alignment Sequence Homology, Amino Acid |
| IF | 1.516 |
| 引用数 | 16 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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