| RRC ID |
1133
|
| Author |
Wauters G, Avesani V, Charlier J, Janssens M, Vaneechoutte M, Delmée M.
|
| Title |
Distribution of nocardia species in clinical samples and their routine rapid identification in the laboratory.
|
| Journal |
J Clin Microbiol
|
| Abstract |
Eighty-six Nocardia strains isolated from clinical samples in Belgium were identified by 16S rRNA gene sequencing. Eighty-three (96%) strains belonged to only six Nocardia species: N. farcinica (38 [44%]), N. nova (19 [22%]), N. cyriacigeorgica (13 [15%]), N. brasiliensis (6 [6.9%]), N. abscessus (5 [5.8%]), and N. paucivorans (2 [2.3%]). A gallery of nine conventional and enzymatic tests was developed for the rapid identification of the most common species isolated during this survey. Pyrrolidonyl aminopeptidase, gamma-glutamyl aminopeptidase, alpha-mannosidase, and alpha-glucosidase were found to be highly discriminating and could be used to develop an identification scheme.
|
| Volume |
43(6)
|
| Pages |
2624-8
|
| Published |
2005-6-1
|
| DOI |
10.1128/JCM.43.6.2624-2628.2005
|
| PII |
43/6/2624
|
| PMID |
15956375
|
| PMC |
PMC1151960
|
| MeSH |
Algorithms
Bacterial Typing Techniques
Belgium / epidemiology
DNA, Bacterial / analysis
DNA, Ribosomal / analysis
Genes, rRNA
Humans
Nocardia / classification*
Nocardia / enzymology
Nocardia / genetics
Nocardia / isolation & purification*
Nocardia Infections / epidemiology*
Nocardia Infections / microbiology
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Species Specificity
Time Factors
|
| IF |
5.897
|
| Times Cited |
59
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| Pathogenic microorganisms |
|
