| RRC ID |
1135
|
| Author |
Sugita T, Takeo K, Hama K, Virtudazo E, Takashima M, Nishikawa A, Kucsera J, Dorogi J, Komori S, Nakagaki K, Vollekova A, Slavikova E, Farkas V.
|
| Title |
DNA sequence diversity of intergenic spacer I region in the non-lipid-dependent species Malassezia pachydermatis isolated from animals.
|
| Journal |
Med Mycol
|
| Abstract |
The non-lipid-dependent species Malassezia pachydermatis is frequently isolated from animals. We analyzed the DNA sequences of the intergenic spacer (IGS) 1 region, which is the most variable region in the rRNA gene, of 43 M. pachydermatis strains obtained from dogs or cats. The lengths of the IGS 1 regions ranged from 552 to 898 bp and, based on the nucleotide sequence, these IGS 1 regions were divided into three major groups with 10 subtypes. Group 1 (552-601 bp long) was characterized by the short sequence repeat (CAGCA)n and had four to 14 repeats, and Group 3 (749-898 bp long), which included the neotype strain of M. pachydermatis, was characterized by the sequence (CAGCATAACATAACACACAACA)n in the IGS1 region. Group 2 possessed partial sequences of both Groups 1 and 3. Each group shared only 41.7-55.4% similarity in the IGS1 region with the other groups. The internal transcribed spacer (ITS) region and D1/D2 26S rDNA in the rRNA gene were also sequenced for representative strains in each IGS group. The groups were distinguished by both ITS (698-712 bp long including 5.8S rDNA) and D1/D2 26S rDNA (624 bp long) sequences with sequence similarities of 91.7-96.0% and 99.7-99.0%, respectively. Our results indicate that the sequence of the IGS region of M. pachydermatis has a remarkable intraspecies diversity, compared with ITS or D1/D2 26S rDNA, and that multiple genotypic strains of M. pachydermatis colonize animal skin.
|
| Volume |
43(1)
|
| Pages |
21-6
|
| Published |
2005-2-1
|
| DOI |
10.1080/1369378042000193185
|
| PMID |
15712605
|
| MeSH |
Animals
Base Sequence
Cat Diseases / microbiology*
Cats
DNA, Fungal / analysis
DNA, Ribosomal Spacer / analysis
DNA, Ribosomal Spacer / genetics*
Dermatomycoses / microbiology
Dermatomycoses / veterinary*
Dog Diseases / microbiology*
Dogs
Genetic Variation*
Malassezia / classification*
Malassezia / genetics
Molecular Sequence Data
RNA, Ribosomal / genetics
Sequence Analysis, DNA
Skin / microbiology
|
| IF |
2.822
|
| Times Cited |
28
|
|
WOS Category
|
VETERINARY SCIENCES
MYCOLOGY
INFECTIOUS DISEASES
|
| Resource |
| Pathogenic microorganisms |
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M. pachydermatis? |
