Reference - Detail
RRC ID | 11387 |
---|---|
Author | Ishii K, Hamamoto H, Imamura K, Adachi T, Shoji M, Nakayama K, Sekimizu K. |
Title | Porphyromonas gingivalis peptidoglycans induce excessive activation of the innate immune system in silkworm larvae. |
Journal | J Biol Chem |
Abstract |
Porphyromonas gingivalis, a pathogen that causes inflammation in human periodontal tissue, killed silkworm (Bombyx mori, Lepidoptera) larvae when injected into the blood (hemolymph). Silkworm lethality was not rescued by antibiotic treatment, and heat-killed bacteria were also lethal. Heat-killed bacteria of mutant P. gingivalis strains lacking virulence factors also killed silkworms. Silkworms died after injection of peptidoglycans purified from P. gingivalis (pPG), and pPG toxicity was blocked by treatment with mutanolysin, a peptidoglycan-degrading enzyme. pPG induced silkworm hemolymph melanization at the same dose as that required to kill the animal. pPG injection increased caspase activity in silkworm tissues. pPG-induced silkworm death was delayed by injecting melanization-inhibiting reagents (a serine protease inhibitor and 1-phenyl-2-thiourea), antioxidants (N-acetyl-l-cysteine, glutathione, and catalase), and a caspase inhibitor (Ac-DEVD-CHO). Thus, pPG induces excessive activation of the innate immune response, which leads to the generation of reactive oxygen species and apoptotic cell death in the host tissue. |
Volume | 285(43) |
Pages | 33338-33347 |
Published | 2010-10-22 |
DOI | 10.1074/jbc.M110.112987 |
PII | S0021-9258(20)47213-8 |
PMID | 20702417 |
PMC | PMC2963355 |
MeSH | Animals Antioxidants / pharmacology Apoptosis / drug effects Apoptosis / immunology Bacteroidaceae Infections / immunology Bacteroidaceae Infections / microbiology Bombyx / immunology* Bombyx / microbiology Cysteine Proteinase Inhibitors / pharmacology Hemolymph / immunology* Hemolymph / microbiology Humans Immunity, Innate / drug effects* Immunity, Innate / physiology Larva / immunology Larva / microbiology Peptidoglycan / immunology* Peptidoglycan / pharmacology Periodontitis / immunology Periodontitis / microbiology Porphyromonas gingivalis / immunology* Porphyromonas gingivalis / pathogenicity Reactive Oxygen Species / immunology Serine Proteinase Inhibitors / pharmacology |
IF | 4.238 |
Times Cited | 33 |
WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
Resource | |
Silkworms | |
General Microbes | JCM 1290 JCM 6425 |