論文 - 詳細
| RRC ID | 11387 |
|---|---|
| 著者 | Ishii K, Hamamoto H, Imamura K, Adachi T, Shoji M, Nakayama K, Sekimizu K. |
| タイトル | Porphyromonas gingivalis peptidoglycans induce excessive activation of the innate immune system in silkworm larvae. |
| ジャーナル | J Biol Chem |
| Abstract |
Porphyromonas gingivalis, a pathogen that causes inflammation in human periodontal tissue, killed silkworm (Bombyx mori, Lepidoptera) larvae when injected into the blood (hemolymph). Silkworm lethality was not rescued by antibiotic treatment, and heat-killed bacteria were also lethal. Heat-killed bacteria of mutant P. gingivalis strains lacking virulence factors also killed silkworms. Silkworms died after injection of peptidoglycans purified from P. gingivalis (pPG), and pPG toxicity was blocked by treatment with mutanolysin, a peptidoglycan-degrading enzyme. pPG induced silkworm hemolymph melanization at the same dose as that required to kill the animal. pPG injection increased caspase activity in silkworm tissues. pPG-induced silkworm death was delayed by injecting melanization-inhibiting reagents (a serine protease inhibitor and 1-phenyl-2-thiourea), antioxidants (N-acetyl-l-cysteine, glutathione, and catalase), and a caspase inhibitor (Ac-DEVD-CHO). Thus, pPG induces excessive activation of the innate immune response, which leads to the generation of reactive oxygen species and apoptotic cell death in the host tissue. |
| 巻・号 | 285(43) |
| ページ | 33338-33347 |
| 公開日 | 2010-10-22 |
| DOI | 10.1074/jbc.M110.112987 |
| PII | S0021-9258(20)47213-8 |
| PMID | 20702417 |
| PMC | PMC2963355 |
| MeSH | Animals Antioxidants / pharmacology Apoptosis / drug effects Apoptosis / immunology Bacteroidaceae Infections / immunology Bacteroidaceae Infections / microbiology Bombyx / immunology* Bombyx / microbiology Cysteine Proteinase Inhibitors / pharmacology Hemolymph / immunology* Hemolymph / microbiology Humans Immunity, Innate / drug effects* Immunity, Innate / physiology Larva / immunology Larva / microbiology Peptidoglycan / immunology* Peptidoglycan / pharmacology Periodontitis / immunology Periodontitis / microbiology Porphyromonas gingivalis / immunology* Porphyromonas gingivalis / pathogenicity Reactive Oxygen Species / immunology Serine Proteinase Inhibitors / pharmacology |
| IF | 4.238 |
| 引用数 | 33 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| カイコ | |
| 一般微生物 | JCM 1290 JCM 6425 |