| RRC ID |
11391
|
| Author |
Kataoka N, Miyake S, Azuma M.
|
| Title |
Aquaporin and aquaglyceroporin in silkworms, differently expressed in the hindgut and midgut of Bombyx mori.
|
| Journal |
Insect Mol Biol
|
| Abstract |
Two cDNAs similar to aquaporins (AQPs) from other insect species were identified and characterized from the silkworm larva, Bombyx mori. The first cDNA (AQP-Bom1) cloned from the anterior silk gland encodes a 25 900 Da protein similar to insect AQPs isolated from several liquid-feeding insects. The second cDNA (AQP-Bom2) cloned from the posterior midgut encodes a 27 694 Da protein. Northern blot analysis has revealed that the AQP-Bom1 mRNA (2.3 kb) is expressed predominantly in the hindgut (colon and rectum), and moderately or minimally in the silk gland, midgut and Malpighian tubules, while the AQP-Bom2 mRNA (1.3 kb) is mainly expressed in the posterior midgut and Malpighian tubules. Functional analysis in Xenopus oocytes microinjected with the cRNA of these AQPs revealed that the AQP-Bom1 mRNA encodes a water-specific aquaporin, likely involved in the water retrieval function of the hindgut, while the AQP-Bom2 mRNA encodes an aquaglyceroporin, increasing glycerol and urea uptake.
|
| Volume |
18(3)
|
| Pages |
303-14
|
| Published |
2009-6-1
|
| DOI |
10.1111/j.1365-2583.2009.00871.x
|
| PII |
IMB871
|
| PMID |
19366375
|
| MeSH |
Amino Acid Sequence
Animals
Aquaglyceroporins / genetics
Aquaglyceroporins / metabolism*
Base Sequence
Bombyx / genetics
Bombyx / metabolism*
Gastrointestinal Tract / metabolism
Glycerol / metabolism
Homeostasis
Larva / metabolism
Molecular Sequence Data
Oocytes
Permeability
RNA, Messenger / metabolism
Sequence Analysis, DNA
Urea / metabolism
Water / metabolism
Xenopus
|
| IF |
2.533
|
| Times Cited |
43
|
|
WOS Category
|
ENTOMOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Silkworms |
|
