RRC ID 11400
Author Vidali L, Burkart GM, Augustine RC, Kerdavid E, Tüzel E, Bezanilla M.
Title Myosin XI is essential for tip growth in Physcomitrella patens.
Journal Plant Cell
Abstract Class XI myosins are plant specific and responsible for cytoplasmic streaming. Because of the large number of myosin XI genes in angiosperms, it has been difficult to determine their precise role, particularly with respect to tip growth. The moss Physcomitrella patens provides an ideal system to study myosin XI function. P. patens has only two myosin XI genes, and these genes encode proteins that are 94% identical to each other. To determine their role in tip growth, we used RNA interference to specifically silence each myosin XI gene using 5' untranslated region sequences. We discovered that the two myosin XI genes are functionally redundant, since silencing of either gene does not affect growth or polarity. However, simultaneous silencing of both myosin XIs results in severely stunted plants composed of small rounded cells. Although similar to the phenotype resulting from silencing of other actin-associated proteins, we show that this phenotype is not due to altered actin dynamics. Consistent with a role in tip growth, we show that a functional, full-length fusion of monomeric enhanced green fluorescent protein (mEGFP) to myosin XI accumulates at a subcortical, apical region of actively growing protonemal cells.
Volume 22(6)
Pages 1868-82
Published 2010-6
DOI 10.1105/tpc.109.073288
PII tpc.109.073288
PMID 20525854
PMC PMC2910981
MeSH 5' Untranslated Regions* Actins / metabolism Bryopsida / genetics* Bryopsida / growth & development DNA, Complementary / genetics Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes, Plant Genetic Complementation Test Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Molecular Sequence Data Mutagenesis, Site-Directed Myosins / genetics Myosins / metabolism* Plant Proteins / genetics Plant Proteins / metabolism* Plant Shoots / genetics Plant Shoots / growth & development* RNA Interference RNA, Plant / genetics
IF 8.228
Times Cited 55
Arabidopsis / Cultured plant cells, genes pdp20423