論文 - 詳細
| RRC ID | 11400 |
|---|---|
| 著者 | Vidali L, Burkart GM, Augustine RC, Kerdavid E, Tüzel E, Bezanilla M. |
| タイトル | Myosin XI is essential for tip growth in Physcomitrella patens. |
| ジャーナル | Plant Cell |
| Abstract |
Class XI myosins are plant specific and responsible for cytoplasmic streaming. Because of the large number of myosin XI genes in angiosperms, it has been difficult to determine their precise role, particularly with respect to tip growth. The moss Physcomitrella patens provides an ideal system to study myosin XI function. P. patens has only two myosin XI genes, and these genes encode proteins that are 94% identical to each other. To determine their role in tip growth, we used RNA interference to specifically silence each myosin XI gene using 5' untranslated region sequences. We discovered that the two myosin XI genes are functionally redundant, since silencing of either gene does not affect growth or polarity. However, simultaneous silencing of both myosin XIs results in severely stunted plants composed of small rounded cells. Although similar to the phenotype resulting from silencing of other actin-associated proteins, we show that this phenotype is not due to altered actin dynamics. Consistent with a role in tip growth, we show that a functional, full-length fusion of monomeric enhanced green fluorescent protein (mEGFP) to myosin XI accumulates at a subcortical, apical region of actively growing protonemal cells. |
| 巻・号 | 22(6) |
| ページ | 1868-82 |
| 公開日 | 2010-6-1 |
| DOI | 10.1105/tpc.109.073288 |
| PII | tpc.109.073288 |
| PMID | 20525854 |
| PMC | PMC2910981 |
| MeSH | 5' Untranslated Regions* Actins / metabolism Bryopsida / genetics* Bryopsida / growth & development DNA, Complementary / genetics Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes, Plant Genetic Complementation Test Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Molecular Sequence Data Mutagenesis, Site-Directed Myosins / genetics Myosins / metabolism* Plant Proteins / genetics Plant Proteins / metabolism* Plant Shoots / genetics Plant Shoots / growth & development* RNA Interference RNA, Plant / genetics |
| IF | 9.618 |
| 引用数 | 84 |
| WOS 分野 | PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pdp20423 |