| RRC ID |
11405
|
| Author |
Shahriari M, Keshavaiah C, Scheuring D, Sabovljevic A, Pimpl P, Häusler RE, Hülskamp M, Schellmann S.
|
| Title |
The AAA-type ATPase AtSKD1 contributes to vacuolar maintenance of Arabidopsis thaliana.
|
| Journal |
Plant J
|
| Abstract |
The vacuole is the most prominent organelle of plant cells. Despite its importance for many physiological and developmental aspects of plant life, little is known about its biogenesis and maintenance. Here we show that Arabidopsis plants expressing a dominant-negative version of the AAA (ATPase associated with various cellular activities) ATPase AtSKD1 (SUPPRESSOR OF K+ TRANSPORT GROWTH DEFECT1) under the control of the trichome-specific GLABRA2 (GL2) promoter exhibit normal vacuolar development in early stages of trichome development. Shortly after its formation, however, the large central vacuole is fragmented and finally disappears completely. Secretion assays with amylase fused to the vacuolar sorting signal of Sporamin show that dominant-negative AtSKD1 inhibits vacuolar trafficking of the reporter that is instead secreted. In addition, trichomes expressing dominant-negative AtSKD1 frequently contain multiple nuclei. Our results suggest that AtSKD1 contributes to vacuolar protein trafficking and thereby to the maintenance of the large central vacuole of plant cells, and might play a role in cell-cycle regulation.
|
| Volume |
64(1)
|
| Pages |
71-85
|
| Published |
2010-10-1
|
| DOI |
10.1111/j.1365-313X.2010.04310.x
|
| PII |
TPJ4310
|
| PMID |
20663085
|
| MeSH |
Adenosine Triphosphatases / genetics
Adenosine Triphosphatases / metabolism*
Arabidopsis / enzymology
Arabidopsis / genetics*
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Cell Nucleus / metabolism
DNA, Plant / genetics
Endosomes / metabolism
Gene Expression Regulation, Plant
Mutagenesis, Site-Directed
Promoter Regions, Genetic
Vacuoles / metabolism*
|
| IF |
6.141
|
| Times Cited |
42
|
|
WOS Category
|
PLANT SCIENCES
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
pda11165
pda02568
pda02482
pda01520
pda11379
pda04291
pda01247
pda07337
pda02603 |
