| RRC ID |
11415
|
| Author |
Ratnayake RM, Inoue H, Nonami H, Akita M.
|
| Title |
Alternative processing of Arabidopsis Hsp70 precursors during protein import into chloroplasts.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
During protein import into chloroplasts, one of the Hsp70 proteins in pea (Hsp70-IAP), previously reported to localize in the intermembrane space of chloroplasts, was found to interact with the translocating precursor protein but the gene for Hsp70-IAP has not been identified yet. In an attempt to identify the Arabidopsis homolog of Hsp70-IAP, we employed an in vitro protein import assay to determine the localization of three Arabidopsis Hsp70 homologs (AtHsp70-6 through 8), predicted for chloroplast targeting. AtHsp70-6 and AtHsp70-7 were imported into chloroplasts and processed into similar-sized mature forms. In addition, a smaller-sized processed form of AtHsp70-6 was observed. All the processed forms of both AtHsp70 proteins were localized in the stroma. Organelle-free processing assays revealed that the larger processed forms of both AtHsp70-6 and AtHsp70-7 were cleaved by stromal processing peptidase, whereas the smaller processed form of AtHsp70-6 was produced by an unspecified peptidase.
|
| Volume |
72(11)
|
| Pages |
2926-35
|
| Published |
2008-11-1
|
| DOI |
10.1271/bbb.80408
|
| PII |
JST.JSTAGE/bbb/80408
|
| PMID |
18997426
|
| MeSH |
Amino Acid Sequence
Animals
Antibodies, Monoclonal / metabolism
Arabidopsis / cytology*
Arabidopsis / metabolism*
Chloroplasts / metabolism*
Computational Biology
Glycine
HSP70 Heat-Shock Proteins / chemistry
HSP70 Heat-Shock Proteins / metabolism*
Humans
Mice
Molecular Sequence Data
Plant Proteins / chemistry
Plant Proteins / metabolism*
Protein Precursors / chemistry
Protein Precursors / metabolism*
Protein Transport
Time Factors
Trypsin / metabolism
|
| IF |
1.516
|
| Times Cited |
27
|
|
WOS Category
|
FOOD SCIENCE & TECHNOLOGY
CHEMISTRY, APPLIED
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
pda07010
pda08520
pda09985 |
