RRC ID 1142
Author Amano M, Ohkusu K, Kusaba K, Ikeda H, Nagasawa Z, Aoki Y, Kawamura Y, Kobatake S, Tanaka T, Matsuura S, Ezaki T.
Title Quantitative microarray-based DNA-DNA hybridization assay for measuring genetic distances among bacterial species and its application to the identification of family Enterobacteriaceae.
Journal Microbiol. Immunol.
Abstract Quantitative DNA-DNA hybridization to measure the genetic distances among bacterial species is indispensable for taxonomical determination. In the current studies, we developed a method to determine bacterial DNA relatedness on a glass microarray. Reference DNAs representing a total 93 species of Enterobacteriaceae were arrayed on a glass microplate, and signal intensities were measured after 2 hr of hybridization with Cy3-labeled bacterial DNAs. All immobilized DNAs from members of the family Enterobacteriaceae were identified by this method except for DNAs from Yersinia pseudotuberculosis and Y. pestis. These results suggest that quantitative microarray hybridization could be an alternative to conventional DNA-DNA hybridization for measuring chromosome relatedness among bacterial species.
Volume 49(3)
Pages 255-63
Published 2005
PII JST.JSTAGE/mandi/49.255
PMID 15781999
MeSH DNA, Bacterial / genetics DNA, Bacterial / isolation & purification Enterobacteriaceae / classification* Enterobacteriaceae / genetics* Escherichia / classification Escherichia / genetics Klebsiella / classification Klebsiella / genetics Nucleic Acid Hybridization Oligonucleotide Array Sequence Analysis / methods* Salmonella / classification Salmonella / genetics Serratia / classification Serratia / genetics Shigella / classification Shigella / genetics Species Specificity Temperature Time Factors
IF 1.442
Times Cited 2
Pathogenic microorganisms Enterobacteriaceae?