RRC ID 11437
著者 Kusano M, Redestig H, Hirai T, Oikawa A, Matsuda F, Fukushima A, Arita M, Watanabe S, Yano M, Hiwasa-Tanase K, Ezura H, Saito K.
タイトル Covering chemical diversity of genetically-modified tomatoes using metabolomics for objective substantial equivalence assessment.
ジャーナル PLoS ONE
Abstract As metabolomics can provide a biochemical snapshot of an organism's phenotype it is a promising approach for charting the unintended effects of genetic modification. A critical obstacle for this application is the inherently limited metabolomic coverage of any single analytical platform. We propose using multiple analytical platforms for the direct acquisition of an interpretable data set of estimable chemical diversity. As an example, we report an application of our multi-platform approach that assesses the substantial equivalence of tomatoes over-expressing the taste-modifying protein miraculin. In combination, the chosen platforms detected compounds that represent 86% of the estimated chemical diversity of the metabolites listed in the LycoCyc database. Following a proof-of-safety approach, we show that % had an acceptable range of variation while simultaneously indicating a reproducible transformation-related metabolic signature. We conclude that multi-platform metabolomics is an approach that is both sensitive and robust and that it constitutes a good starting point for characterizing genetically modified organisms.
巻・号 6(2)
ページ e16989
公開日 2011-3-2
DOI 10.1371/journal.pone.0016989
PMID 21359231
PMC PMC3040210
MeSH Algorithms Food Safety / methods Food, Genetically Modified* Gene Expression Regulation, Plant Glycoproteins / genetics Lycopersicon esculentum / chemistry* Lycopersicon esculentum / genetics Lycopersicon esculentum / metabolism Metabolome Metabolomics* / methods Models, Biological Nutritive Value Plants, Genetically Modified / chemistry Plants, Genetically Modified / genetics Plants, Genetically Modified / metabolism Quality Control Taste / physiology
IF 2.806
引用数 44
トマト TOMJPF00003(Aichi-first) TOMJPF00004(Ailsa Craig) TOMJPF00001 TOMJPF00002(Moneymaker) TOMJPF00005(M82) TOMJPF00006(Rutgers)