RRC ID |
11437
|
Author |
Kusano M, Redestig H, Hirai T, Oikawa A, Matsuda F, Fukushima A, Arita M, Watanabe S, Yano M, Hiwasa-Tanase K, Ezura H, Saito K.
|
Title |
Covering chemical diversity of genetically-modified tomatoes using metabolomics for objective substantial equivalence assessment.
|
Journal |
PLoS One
|
Abstract |
As metabolomics can provide a biochemical snapshot of an organism's phenotype it is a promising approach for charting the unintended effects of genetic modification. A critical obstacle for this application is the inherently limited metabolomic coverage of any single analytical platform. We propose using multiple analytical platforms for the direct acquisition of an interpretable data set of estimable chemical diversity. As an example, we report an application of our multi-platform approach that assesses the substantial equivalence of tomatoes over-expressing the taste-modifying protein miraculin. In combination, the chosen platforms detected compounds that represent 86% of the estimated chemical diversity of the metabolites listed in the LycoCyc database. Following a proof-of-safety approach, we show that % had an acceptable range of variation while simultaneously indicating a reproducible transformation-related metabolic signature. We conclude that multi-platform metabolomics is an approach that is both sensitive and robust and that it constitutes a good starting point for characterizing genetically modified organisms.
|
Volume |
6(2)
|
Pages |
e16989
|
Published |
2011-2-16
|
DOI |
10.1371/journal.pone.0016989
|
PMID |
21359231
|
PMC |
PMC3040210
|
MeSH |
Algorithms
Food Safety / methods
Food, Genetically Modified*
Gene Expression Regulation, Plant
Glycoproteins / genetics
Lycopersicon esculentum / chemistry*
Lycopersicon esculentum / genetics
Lycopersicon esculentum / metabolism
Metabolome
Metabolomics* / methods
Models, Biological
Nutritive Value
Plants, Genetically Modified / chemistry
Plants, Genetically Modified / genetics
Plants, Genetically Modified / metabolism
Quality Control
Taste / physiology
|
IF |
2.74
|
Times Cited |
65
|
WOS Category
|
PLANT SCIENCES
|
Resource |
Tomato |
TOMJPF00003(Aichi-first)
TOMJPF00004(Ailsa Craig)
TOMJPF00001
TOMJPF00002(Moneymaker)
TOMJPF00005(M82)
TOMJPF00006(Rutgers) |