Reference - Detail
| RRC ID | 11439 |
|---|---|
| Author | Suehiro Y, Kinoshita M, Okuyama T, Shimada A, Naruse K, Takeda H, Kubo T, Hashimoto M, Takeuchi H. |
| Title | Transient and permanent gene transfer into the brain of the teleost fish medaka (Oryzias latipes) using human adenovirus and the Cre-loxP system. |
| Journal | FEBS Lett |
| Abstract |
In this study, we demonstrated that human type-5 adenovirus infected the brain of the teleost fish, medaka (Oryzias latipes), in vivo. Injection of adenoviral vector into the mesencephalic ventricle of medaka larvae induced the expression of reporter genes in some parts of the telencephalon, the periventricular area of the mesencephalon and diencephalon, and the cerebellum. Additionally, the Cre-loxP system works in medaka brains using transgenic medaka carrying a vector containing DsRed2, flanked by loxP sites under control of the beta-actin promoter and downstream promoterless enhanced green fluorescent protein (EGFP). We demonstrated that the presence of green fluorescence depended on injection of adenoviral vector expressing the Cre gene and confirmed that EGFP mRNA was transcribed in the virus-injected larvae. |
| Volume | 584(16) |
| Pages | 3545-9 |
| Published | 2010-8-20 |
| DOI | 10.1016/j.febslet.2010.06.047 |
| PII | S0014-5793(10)00558-2 |
| PMID | 20621097 |
| MeSH | Adenoviruses, Human / genetics* Animals Animals, Genetically Modified Brain / anatomy & histology Brain / metabolism* Brain / virology* Gene Expression Gene Transfer Techniques* Genes, Reporter Genetic Vectors Humans Integrases Luminescent Proteins / genetics Oryzias / anatomy & histology Oryzias / genetics* Oryzias / virology* Recombinant Proteins / genetics |
| IF | 3.057 |
| Times Cited | 6 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Medaka | MT837 (d-rR/TOKYO) TG861 (d-rR-Tg (beta-actin-loxP-DsRed2-loxP-GFP)) |