Reference - Detail
RRC ID | 11446 |
---|---|
Author | Koga A, Sasaki S, Naruse K, Shimada A, Sakaizumi M. |
Title | Occurrence of a short variant of the Tol2 transposable element in natural populations of the medaka fish. |
Journal | Genet Res (Camb) |
Abstract |
Tol2 is a member of the hAT (hobo/Activator/Tam3) transposable element family, residing as 10-30 copies per diploid genome in the medaka fish. We previously reported that this element is highly homogeneous in structure at both the restriction map level and the nucleotide sequence level. It was, however, possible that there is variation of such a low frequency as not to have been detected in our previous surveys, in which samples from 12 geographical locations were used. In the present study, we first conducted searches of genome sequence databases of medaka, and found a 119-bp-long internal deletion. We then conducted a survey of samples from 58 locations for this deletion by performing PCR preceded by restriction enzyme digestion to increase the sensitivity to this deletion. We found that copies suffering this deletion have spread, or have been generated by multiple origins, in the northern-to-central part of mainland Japan. Thus, although the high homogeneity in structure is a distinct feature of Tol2, variation does exist at low frequencies in natural populations of medaka. The current status of Tol2 is expected to provide information with which results of future surveys can be compared for clarification of determinants of population dynamics of this DNA-based element. |
Volume | 93(1) |
Pages | 13-21 |
Published | 2011-2-1 |
DOI | 10.1017/S0016672310000479 |
PII | S0016672310000479 |
PMID | 21134318 |
MeSH | Animals Base Sequence DNA Transposable Elements / genetics* Genome Molecular Sequence Data Oryzias / genetics* Transposases / genetics* |
IF | 1.167 |
Times Cited | 1 |
WOS Category | GENETICS & HEREDITY |
Resource | |
Medaka | WS207(Higashidori) WS208(Honjo) Yokote Kajikawa Niigata-Tayuhama Niitsu Shirone WS210(Teradomari) WS212(Ojiya) WS211(Hamochi) WS214(Nanao) IB833(Kaga) WS215(Maizuru) WS216(Ine) WS217(Kumihama (Nagae)) WS221(Toyooka) Kasumi WS222(Hamasaka) WS223(Hanamaki) WS225(Sendai) WS226(Kawachi) WS227(Mito) WS231(Odawara) WS233(Fuji) WS3(Nagoya) WS234(Saori) WS238(Hikone) WS236(Kumano) Kobe WS242(Saigo) WS245(Miyoshi) WS244(Hohoku) WS247(Iwakuni) WS246(Kamiura) WS237(Aki) Tosanakamura WS248(Saiki) WS249(Saito) WS250(Ashibe) WS251(Kashima) WS252(Sato) WS255(Ginoza) WS257(Sokcho) Toseong Sachon Guoje Kwangi WS261(Jindo) Sinpyong WS266(Samsan) WS265(Guhang) Buyong Simcheon WS258(Daebu) Paltan Shanghai WS262(Ilan) WS263(Kunming) |