RRC ID 11451
著者 Wu SY, Lai MC.
タイトル Methanogenic archaea isolated from Taiwan's Chelungpu fault.
ジャーナル Appl Environ Microbiol
Abstract Terrestrial rocks, petroleum reservoirs, faults, coal seams, and subseafloor gas hydrates contain an abundance of diverse methanoarchaea. However, reports on the isolation, purification, and characterization of methanoarchaea in the subsurface environment are rare. Currently, no studies investigating methanoarchaea within fault environments exist. In this report, we succeeded in obtaining two new methanogen isolates, St545Mb(T) of newly proposed species Methanolobus chelungpuianus and Methanobacterium palustre FG694aF, from the Chelungpu fault, which is the fault that caused a devastating earthquake in central Taiwan in 1999. Strain FG694aF was isolated from a fault gouge sample obtained at 694 m below land surface (mbls) and is an autotrophic, mesophilic, nonmotile, thin, filamentous-rod-shaped organism capable of using H(2)-CO(2) and formate as substrates for methanogenesis. The morphological, biochemical, and physiological characteristics and 16S rRNA gene sequence analysis revealed that this isolate belongs to Methanobacterium palustre. The mesophilic strain St545Mb(T), isolated from a sandstone sample at 545 mbls, is a nonmotile, irregular, coccoid organism that uses methanol and trimethylamine as substrates for methanogenesis. The 16S rRNA gene sequence of strain St545Mb(T) was 99.0% similar to that of Methanolobus psychrophilus strain R15 and was 96 to 97.5% similar to the those of other Methanolobus species. However, the optimal growth temperature and total cell protein profile of strain St545Mb(T) were different from those of M. psychrophilus strain R15, and whole-genome DNA-DNA hybridization revealed less than 20% relatedness between these two strains. On the basis of these observations, we propose that strain St545Mb(T) (DSM 19953(T); BCRC AR10030; JCM 15159) be named Methanolobus chelungpuianus sp. nov. Moreover, the environmental DNA database survey indicates that both Methanolobus chelungpuianus and Methanobacterium palustre are widespread in the subsurface environment.
巻・号 77(3)
ページ 830-8
公開日 2011-2-1
DOI 10.1128/AEM.01539-10
PII AEM.01539-10
PMID 21148697
PMC PMC3028716
MeSH Archaeal Proteins / analysis Base Composition Culture Media Earthquakes* Genes, rRNA Geologic Sediments / microbiology* Methane / metabolism* Methanobacterium / classification* Methanobacterium / genetics Methanobacterium / isolation & purification* Methanobacterium / physiology Methanosarcinaceae / classification* Methanosarcinaceae / genetics Methanosarcinaceae / isolation & purification* Methanosarcinaceae / physiology Molecular Sequence Data Nucleic Acid Hybridization Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Taiwan
IF 4.016
引用数 17
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
一般微生物 JCM 15159 JCM 15160