Reference - Detail
RRC ID | 11487 |
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Author | Bräuer SL, Cadillo-Quiroz H, Ward RJ, Yavitt JB, Zinder SH. |
Title | Methanoregula boonei gen. nov., sp. nov., an acidiphilic methanogen isolated from an acidic peat bog. |
Journal | Int J Syst Evol Microbiol |
Abstract |
A novel acidiphilic, hydrogenotrophic methanogen, designated strain 6A8(T), was isolated from an acidic (pH 4.0-4.5) and ombrotrophic (rain-fed) bog located near Ithaca, NY, USA. Cultures were dimorphic, containing thin rods (0.2-0.3 μm in diameter and 0.8-3.0 μm long) and irregular cocci (0.2-0.8 μm in diameter). The culture utilized H(2)/CO(2) to produce methane but did not utilize formate, acetate, methanol, ethanol, 2-propanol, butanol or trimethylamine. Optimal growth conditions were near pH 5.1 and 35 °C. The culture grew in basal medium containing as little as 0.43 mM Na(+) and growth was inhibited completely by 50 mM NaCl. To our knowledge, strain 6A8(T) is one of the most acidiphilic (lowest pH optimum) and salt-sensitive methanogens in pure culture. Acetate, coenzyme M, vitamins and yeast extract were required for growth. It is proposed that a new genus and species be established for this organism, Methanoregula boonei gen. nov., sp. nov. The type strain of Methanoregula boonei is 6A8(T) (=DSM 21154(T) =JCM 14090(T)). |
Volume | 61(Pt 1) |
Pages | 45-52 |
Published | 2011-1-1 |
DOI | 10.1099/ijs.0.021782-0 |
PMID | 20154331 |
MeSH | Carbon Dioxide / metabolism Cluster Analysis Culture Media / chemistry DNA, Archaeal / chemistry DNA, Archaeal / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Environmental Microbiology* Hydrogen / metabolism Hydrogen-Ion Concentration Methane / metabolism Methanomicrobiales / classification* Methanomicrobiales / genetics Methanomicrobiales / isolation & purification* Methanomicrobiales / physiology Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sodium Chloride / metabolism Soil Temperature United States Wetlands |
IF | 2.415 |
Times Cited | 93 |
WOS Category | MICROBIOLOGY |
Resource | |
General Microbes | JCM 14090 |