論文 - 詳細
| RRC ID | 11513 |
|---|---|
| 著者 | Asahara T, Shimizu K, Takada T, Kado S, Yuki N, Morotomi M, Tanaka R, Nomoto K. |
| タイトル | Protective effect of Lactobacillus casei strain Shirota against lethal infection with multi-drug resistant Salmonella enterica serovar Typhimurium DT104 in mice. |
| ジャーナル | J Appl Microbiol |
| Abstract |
AIMS:The anti-infectious activity of lactobacilli against multi-drug resistant Salmonella enterica serovar Typhimurium DT104 (DT104) was examined in a murine model of an opportunistic antibiotic-induced infection. METHODS AND RESULTS:Explosive intestinal growth and subsequent lethal extra-intestinal translocation after oral infection with DT104 during fosfomycin (FOM) administration was significantly inhibited by continuous oral administration of Lactobacillus casei strain Shirota (LcS), which is naturally resistant to FOM, at a dose of 10(8) colony-forming units per mouse daily to mice. Comparison of the anti-Salmonella activity of several Lactobacillus type strains with natural resistance to FOM revealed that Lactobacillus brevis ATCC 14869(T) , Lactobacillus plantarum ATCC 14917(T) , Lactobacillus reuteri JCM 1112(T) , Lactobacillus rhamnosus ATCC 7469(T) and Lactobacillus salivarius ATCC 11741(T) conferred no activity even when they obtained the high population levels almost similar to those of the effective strains such as LcS, Lact. casei ATCC 334(T) and Lactobacillus zeae ATCC 15820(T) . The increase in concentration of organic acids and maintenance of the lower pH in the intestine because of Lactobacillus colonization were correlated with the anti-infectious activity. Moreover, heat-killed LcS was not protective against the infection, suggesting that the metabolic activity of lactobacilli is important for the anti-infectious activity. CONCLUSION:These results suggest that certain lactobacilli in combination with antibiotics may be useful for prophylaxis against opportunistic intestinal infections by multi-drug resistant pathogens, such as DT104. SIGNIFICANCE AND IMPACT OF THE STUDY:Antibiotics such as FOM disrupt the metabolic activity of the intestinal microbiota that produce organic acids, and that only probiotic strains that are metabolically active in vivo should be selected to prevent intestinal infection when used clinically in combination with certain antibiotics. |
| 巻・号 | 110(1) |
| ページ | 163-73 |
| 公開日 | 2011-1-1 |
| DOI | 10.1111/j.1365-2672.2010.04884.x |
| PMID | 21059159 |
| MeSH | Acetic Acid / pharmacology Animals Disease Models, Animal Drug Resistance, Multiple, Bacterial Lactobacillus Lactobacillus casei* Male Mice Mice, Inbred BALB C Probiotics / pharmacology Probiotics / therapeutic use* Salmonella Infections, Animal / pathology Salmonella Infections, Animal / prevention & control* Salmonella typhimurium* / drug effects Salmonella typhimurium* / growth & development |
| IF | 3.066 |
| 引用数 | 19 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 1112 |