Reference - Detail
| RRC ID | 11525 |
|---|---|
| Author | Ryuda N, Hashimoto T, Ueno D, Inoue K, Someya T. |
| Title | Visualization and direct counting of individual denitrifying bacterial cells in soil by nirK-targeted direct in situ PCR. |
| Journal | Microbes Environ |
| Abstract |
The abundance of denitrifying bacteria in soil has been determined primarily by the conventional most probable number (MPN) method. We have developed a single-cell identification technique that is culture-independent, direct in situ PCR, to enumerate denitrifying bacteria in soils. The specificity of this method was evaluated with six species of denitrifying bacteria using nirK as the target gene; Escherichia coli was used as a negative control. Almost all (97.3%-100%) of the nirK-type denitrifying bacteria (Agromonas oligotrophica, Alcaligenes faecalis, Achromobacter denitrificans, Bradyrhizobium japonicum, and Pseudomonas chlororaphis) were detected by direct in situ PCR, whereas no E. coli cells and only a few cells (2.4%) of nirS-type denitrifying bacteria (Pseudomonas aeruginosa) were detected. Numbers of denitrifying bacteria in upland and paddy soil samples quantified by this method were 3.3 × 10(8) to 2.6 × 10(9) cells g(-1) dry soil. These values are approximately 1,000 to 300,000 times higher than those estimated by the MPN method. These results suggest that direct in situ PCR is a better tool for quantifying denitrifying bacteria in soil than the conventional MPN method. |
| Volume | 26(1) |
| Pages | 74-80 |
| Published | 2011-1-1 |
| DOI | 10.1264/jsme2.me10180 |
| PII | JST.JSTAGE/jsme2/ME10180 |
| PMID | 21487206 |
| MeSH | Bacteria / classification Bacteria / genetics* Bacteria / growth & development* Bacteria / isolation & purification Bacterial Proteins / genetics* Bacterial Proteins / metabolism Molecular Sequence Data Nitrite Reductases / genetics* Nitrite Reductases / metabolism Nitrites / metabolism Polymerase Chain Reaction / methods* Soil Microbiology* |
| IF | 2.575 |
| Times Cited | 10 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM 1494 |