| RRC ID |
11581
|
| Author |
Walser JC, Chen B, Feder ME.
|
| Title |
Heat-shock promoters: targets for evolution by P transposable elements in Drosophila.
|
| Journal |
PLoS Genet
|
| Abstract |
Transposable elements are potent agents of genomic change during evolution, but require access to chromatin for insertion-and not all genes provide equivalent access. To test whether the regulatory features of heat-shock genes render their proximal promoters especially susceptible to the insertion of transposable elements in nature, we conducted an unbiased screen of the proximal promoters of 18 heat-shock genes in 48 natural populations of Drosophila. More than 200 distinctive transposable elements had inserted into these promoters; greater than 96% are P elements. By contrast, few or no P element insertions segregate in natural populations in a "negative control" set of proximal promoters lacking the distinctive regulatory features of heat-shock genes. P element transpositions into these same genes during laboratory mutagenesis recapitulate these findings. The natural P element insertions cluster in specific sites in the promoters, with up to eight populations exhibiting P element insertions at the same position; laboratory insertions are into similar sites. By contrast, a "positive control" set of promoters resembling heat-shock promoters in regulatory features harbors few P element insertions in nature, but many insertions after experimental transposition in the laboratory. We conclude that the distinctive regulatory features that typify heat-shock genes (in Drosophila) are especially prone to mutagenesis via P elements in nature. Thus in nature, P elements create significant and distinctive variation in heat-shock genes, upon which evolutionary processes may act.
|
| Volume |
2(10)
|
| Pages |
e165
|
| Published |
2006-10-6
|
| DOI |
10.1371/journal.pgen.0020165
|
| PII |
06-PLGE-RA-0200R2
|
| PMID |
17029562
|
| PMC |
PMC1592238
|
| MeSH |
Animals
DNA Mutational Analysis
DNA Transposable Elements / genetics*
Drosophila melanogaster / genetics*
Evolution, Molecular*
Gene Frequency
Genes, Insect
Geography
Heat-Shock Proteins / genetics*
Mutagenesis, Insertional
Promoter Regions, Genetic / genetics*
Recombination, Genetic
|
| IF |
5.175
|
| Times Cited |
46
|
|
WOS Category
|
GENETICS & HEREDITY
|
| Resource |
| Drosophila |
|
