論文 - 詳細
| RRC ID | 11655 |
|---|---|
| 著者 | Djiane A, Mlodzik M. |
| タイトル | The Drosophila GIPC homologue can modulate myosin based processes and planar cell polarity but is not essential for development. |
| ジャーナル | PLoS One |
| Abstract |
Epithelia often show, in addition to the ubiquitous apico-basal (A/B) axis, a polarization within the plane of the epithelium, perpendicular to the A/B axis. Such planar cell polarity (PCP) is for example evident in the regular arrangement of the stereocilia in the cochlea of the mammalian inner ear or in (almost) all Drosophila adult external structures. GIPCs (GAIP interacting protein, C terminus) were first identified in mammals and bind to the Galphai GTPase activating protein RGS-GAIP. They have been proposed to act in a G-protein coupled complex controlling vesicular trafficking. Although GIPCs have been found to bind to numerous proteins including Frizzled receptors, which participate in PCP establishment, there is little in vivo evidence for the functional role(s) of GIPCs. We show here that overexpressed Drosophila dGIPC alters PCP generation in the wing. We were however unable to find any binding between dGIPC and the Drosophila receptors Fz1 and Fz2. The effect of overexpressed dGIPC is likely due to an effect on the actin cytoskeleton via myosins, since it is almost entirely suppressed by removing a genomic copy of the Myosin VI/jaguar gene. Surprisingly, although dGIPC can interfere with PCP generation and myosin based processes, the complete loss-of-function of dGIPC gives viable adults with no PCP or other detectable defects arguing for a non-essential role of dGIPC in viability and normal Drosophila development. |
| 巻・号 | 5(6) |
| ページ | e11228 |
| 公開日 | 2010-6-21 |
| DOI | 10.1371/journal.pone.0011228 |
| PMID | 20574526 |
| PMC | PMC2888583 |
| MeSH | Actins / metabolism Animals Carrier Proteins / chemistry Carrier Proteins / genetics Carrier Proteins / metabolism* Cell Polarity* Cytoskeleton / metabolism Drosophila Proteins / chemistry Drosophila Proteins / deficiency Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / cytology* Drosophila melanogaster / genetics Drosophila melanogaster / growth & development Drosophila melanogaster / metabolism* Female Gene Expression Regulation Male Membrane Proteins / genetics Myosin Heavy Chains / genetics Myosins / genetics Myosins / metabolism* Sequence Deletion Sequence Homology, Amino Acid* Wings, Animal / cytology Wings, Animal / metabolism |
| IF | 2.74 |
| 引用数 | 14 |
| WOS 分野 | CELL BIOLOGY |
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| 各媒体での言及数の合計 | 0 |
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