Reference - Detail
| RRC ID | 11658 |
|---|---|
| Author | Goto A, Yano T, Terashima J, Iwashita S, Oshima Y, Kurata S. |
| Title | Cooperative regulation of the induction of the novel antibacterial Listericin by peptidoglycan recognition protein LE and the JAK-STAT pathway. |
| Journal | J Biol Chem |
| Abstract |
Intracellular bacteria cause serious infectious diseases such as tuberculosis, shigellosis, and listeriosis. The Drosophila peptidoglycan recognition protein (PGRP)-LE functions as an important host pattern recognition receptor against intracellular bacteria such as Listeria monocytogenes. One PGRP-LE-mediated intracellular response against L. monocytogenes infection is the induction of autophagy, a conserved intracellular degradation system. Here, to further elucidate PGRP-LE-mediated intracellular innate immune responses, we performed a strategic microarray analysis and identified the Listericin gene, whose expression is induced in response to L. monocytogenes infection in a PGRP-LE-dependent manner. RNA interference and overexpression experiments demonstrated that Listericin gene induction is cooperatively regulated by PGRP-LE and the JAK-STAT (Janus kinase-signal transducers and activators of transcription) pathway. An in vitro cell culture assay showed that Listericin is secreted as processed forms and suppresses the growth of L. monocytogenes and Gram-negative bacteria. A colony formation unit assay clearly demonstrated that induction of the Listericin gene suppresses not only the growth of L. monocytogenes but also the growth of Gram-negative bacteria in vivo. Based on these findings, we propose that the Listericin gene encodes a novel antibacterial peptide-like protein whose induction is cooperatively regulated by PGRP-LE and the JAK-STAT pathway. |
| Volume | 285(21) |
| Pages | 15731-8 |
| Published | 2010-5-21 |
| DOI | 10.1074/jbc.M109.082115 |
| PII | S0021-9258(20)49383-4 |
| PMID | 20348097 |
| PMC | PMC2871439 |
| MeSH | Animals Anti-Infective Agents / metabolism* Autophagy / genetics Carrier Proteins / genetics Carrier Proteins / metabolism* Cell Line Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster Janus Kinases / genetics Janus Kinases / metabolism* Listeria monocytogenes / metabolism* Listeriosis / genetics Listeriosis / metabolism* STAT Transcription Factors / genetics STAT Transcription Factors / metabolism* Transcription Factors / genetics Transcription Factors / metabolism* |
| IF | 4.238 |
| Times Cited | 27 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Wikipedia |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | |