RRC ID 11708
Author Koto A, Kuranaga E, Miura M.
Title Apoptosis ensures spacing pattern formation of Drosophila sensory organs.
Journal Curr. Biol.
Abstract BACKGROUND:In both vertebrates and invertebrates, developing organs and tissues must be precisely patterned. One patterning mechanism is Notch/Delta-mediated lateral inhibition. Through the process of lateral inhibition, Drosophila sensory organ precursors (SOPs) are selected and sensory bristles form into a regular pattern. SOP cell fate is determined by high Delta expression and following expression of neurogenic genes like neuralized. SOP selection is spatially and temporally regulated; however, the dynamic process of precise pattern formation is not clearly understood.
RESULTS:In this study, using live-imaging analysis, we show that the appearance of neuralized-positive cells is random in both timing and position. Excess neuralized-positive cells are produced by developmental errors at several steps preceding and accompanying lateral inhibition. About 20% of the neuralized-positive cells show aberrant cell characteristics and high Notch activation, which not only suppress neural differentiation but also induce caspase-dependent cell death. These cells never develop into sensory organs, nor do they disturb bristle patterning.
CONCLUSIONS:Our study reveals the incidence of developmental errors that produce excess neuralized-positive cells during sensory organ development. Notch activation in neuralized-positive cells determines aberrant cell fate and typically induces caspase-dependent cell death. Apoptosis is utilized as a mechanism to remove cells that start neural differentiation at aberrant positions and timing and to ensure robust spacing pattern formation.
Volume 21(4)
Pages 278-87
Published 2011-2-22
DOI 10.1016/j.cub.2011.01.015
PII S0960-9822(11)00038-8
PMID 21276725
MeSH Animals Antibodies Apoptosis / physiology* Body Patterning / physiology* Drosophila / growth & development* Drosophila Proteins / genetics Drosophila Proteins / metabolism Gene Expression Regulation, Developmental Pupa / growth & development Receptors, Notch / genetics Receptors, Notch / metabolism Sense Organs / growth & development*
IF 9.251
Times Cited 15
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
Drosophila