| RRC ID |
11732
|
| 著者 |
Kawasaki S, Ebara S, Nakayama K, Takaoka K.
|
| タイトル |
The E-Box motif, recognized by tissue-specific nuclear factor(s), is important for BMP-4 gene expression in osteogenic cells.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
We analyzed the promoter activity of mouse BMP-4 gene in three different types of cell lines and demonstrated that position -786 to -691 of the 5'-flanking region of exon I plays a critical role for regulation of tissue specific expression of mouse BMP-4 gene in cells of an osteogenic lineage. By use of site-directed mutagenesis, we have established that the E-box, CATCTG, located within this 5'-flanking region, is essential for tissue-specific transcriptional activation of mouse BMP-4 gene and demonstrated that an osteogenic lineage-specific novel transcriptional factor(s) recognizes this E-box.
|
| 巻・号 |
263(2)
|
| ページ |
560-5
|
| 公開日 |
1999-9-24
|
| DOI |
10.1006/bbrc.1999.1402
|
| PII |
S0006-291X(99)91402-X
|
| PMID |
10491331
|
| MeSH |
Animals
Binding, Competitive
Bone Morphogenetic Protein 4
Bone Morphogenetic Proteins / biosynthesis
Bone Morphogenetic Proteins / genetics*
Cell Line
Cell Lineage
DNA Mutational Analysis
Gene Expression Regulation
Mice
Nuclear Proteins / metabolism*
Osteogenesis / physiology*
Promoter Regions, Genetic*
Protein Binding
Tissue Distribution
Transcription Factors / metabolism*
|
| IF |
3.152
|
| リソース情報 |
| ヒト・動物細胞 |
10T1/2(RCB0247)
MC3T3-E1(RCB1126) |
