Reference - Detail
| RRC ID | 11765 |
|---|---|
| Author | Okita K, Hong H, Takahashi K, Yamanaka S. |
| Title | Generation of mouse-induced pluripotent stem cells with plasmid vectors. |
| Journal | Nat Protoc |
| Abstract |
Reprogramming of somatic cells into pluripotent stem cells has been reported by introducing a combination of several transcription factors (Oct3/4, Sox2, Klf4 and c-Myc). The induced pluripotent stem (iPS) cells from patient's somatic cells could be a useful source for drug discovery and cell transplantation therapies. However, to date, most iPS cells were made using viral vectors, such as retroviruses and lentiviruses. Here we describe an alternative method to generate iPS cells from mouse embryonic fibroblasts (MEFs) by continual transfection of plasmid vectors. This protocol takes around 2 months to complete, from MEF isolation to iPS cell establishment. Although the reprogramming efficiency of this protocol is still low, the established iPS cells are most likely free from plasmid integration. This virus-free technique reduces the safety concern for iPS cell generation and application, and provides a source of cells for the investigation of the mechanisms underlying reprogramming and pluripotency. |
| Volume | 5(3) |
| Pages | 418-28 |
| Published | 2010-3-1 |
| DOI | 10.1038/nprot.2009.231 |
| PII | nprot.2009.231 |
| PMID | 20203661 |
| MeSH | Animals Base Sequence Cell Dedifferentiation / genetics* DNA Primers / genetics Embryonic Stem Cells / cytology Embryonic Stem Cells / metabolism Fibroblasts / cytology Genes, myc Genetic Vectors* Humans Kruppel-Like Factor 4 Kruppel-Like Transcription Factors / genetics Mice Octamer Transcription Factor-3 / genetics Plasmids / genetics* Pluripotent Stem Cells / cytology* Pluripotent Stem Cells / metabolism SOXB1 Transcription Factors / genetics Transfection |
| IF | 10.419 |
| Times Cited | 149 |
| WOS Category | BIOCHEMICAL RESEARCH METHODS |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 45 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| Mice | RBRC02290 |