RRC ID |
11783
|
著者 |
Phillips R, Svensson M, Aziz N, Maroof A, Brown N, Beattie L, Signoret N, Kaye PM.
|
タイトル |
Innate killing of Leishmania donovani by macrophages of the splenic marginal zone requires IRF-7.
|
ジャーナル |
PLoS Pathog
|
Abstract |
Highly phagocytic macrophages line the marginal zone (MZ) of the spleen and the lymph node subcapsular sinus. Although these macrophages have been attributed with a variety of functions, including the uptake and clearance of blood and lymph-borne pathogens, little is known about the effector mechanisms they employ after pathogen uptake. Here, we have combined gene expression profiling and RNAi using a stromal macrophage cell line with in situ analysis of the leishmanicidal activity of marginal zone macrophages (MZM) and marginal metallophilic macrophages (MMM) in wild type and gene targeted mice. Our data demonstrate a critical role for interferon regulatory factor-7 (IRF-7) in regulating the killing of intracellular Leishmania donovani by these specialised splenic macrophage sub-populations. This study, therefore, identifies a new role for IRF-7 as a regulator of innate microbicidal activity against this, and perhaps other, non-viral intracellular pathogens. This study also highlights the importance of selecting appropriate macrophage populations when studying pathogen interactions with this functionally diverse lineage of cells.
|
巻・号 |
6(3)
|
ページ |
e1000813
|
公開日 |
2010-3-12
|
DOI |
10.1371/journal.ppat.1000813
|
PMID |
20300600
|
PMC |
PMC2837405
|
MeSH |
Animals
Gene Expression / immunology
Interferon Regulatory Factor-7 / genetics
Interferon Regulatory Factor-7 / immunology*
Interferon Regulatory Factor-7 / metabolism
Interferon-alpha / immunology
Interferon-alpha / metabolism
Interferon-gamma / immunology
Interferon-gamma / metabolism
Leishmania donovani / immunology*
Leishmaniasis, Visceral / immunology*
Macrophages / cytology
Macrophages / immunology*
Macrophages / parasitology*
Mice
Mice, Inbred C57BL
Nitric Oxide / metabolism
Phagosomes / immunology
RNA, Small Interfering
Spleen / immunology
Spleen / parasitology
|
IF |
6.218
|
引用数 |
45
|
WOS 分野
|
PARASITOLOGY
MICROBIOLOGY
VIROLOGY
|
リソース情報 |
実験動物マウス |
RBRC01420 |